C-terminal truncated lipase GD-95 from Geobacillus sp. 95
Enzyme Description
Sequence
MKCCRVMFVLLGLWFVFGLSVPGGRAEAAVSRANDAPIVLLHGFTGWGREEMFGFKYWGGVRGDIEQWLNDNGYQAYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRIHIIAHSQGGQTARMLVSLLENGSQEEREYAKAHNVSLSPLFEGGHRFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLEAAAVASNAPYTSEIYDFKLDQWGLRREPGESFDHYFERLKRSPVWTSTDTARYDLSVPGAEKLNQWVQASPNTYYLSFSTERTYRGALTGNYYPELGMNAFSAVVCAPFLGSYRNPTLGIDDRWLENDGIVNTVSMNGPKRGSSDRIVPYDGALKKGVWNDMGTYNVDHLEIIGVDPNPSFDIRAFYLR
Renata GudiukaitΖΓ³ et al. (2013)
β Influence of N- and/or C-terminal regions on activity, expression, characteristics and structure of lipase from Geobacillus sp. 95
Extremophiles
Β· doi:10.1007/s00792-013-0605-x β
Β· Stability - Incubation
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Database ID
UniProt: V5JF58 β
Sequence Annotation
Explicit - Provided GenBank Accession Number (10 last residues from UniProt sequence absent from the mature protein)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (7 measurements)
7 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 25% | 100 | 59 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Dimethylformamide (DMF) | 25% | 100 | 124 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Ethanol | 25% | 100 | 107 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 25% | 100 | 116 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetone | 25% | 100 | 159 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Isopropanol | 25% | 100 | 172 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Butanol | 25% | 100 | 94 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.