Subtilisin from Bacillus subtilis VTCC-DVN-12-01

Enzyme Description

Species
Bacillus subtilis VTCC-DVN-12-01 β†—
Extremophile
No
EC Number
3.4.21.62 β†—

Sequence

Length: 275 amino acids
AQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPSGSTALKTVVDKAVSSGIVVAAAAGNEGSSGSSSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ
Thao Thi Nguyen et al. (2013) β€” Cloning and enhancing production of a detergent- and organic-solvent-resistant nattokinase from Bacillus subtilis VTCC-DVN-12-01 by using an eight-protease-gene-deficient Bacillus subtilis WB800
Microbial Cell Factories  Β· doi:10.1186/1475-2859-12-79 β†—  Β· Stability - Incubation
12 measurements ▼
Database ID
UniProt: A0FLP4 β†—
Sequence Annotation
Explicit - Provided (106 first residues from UniProt sequence absent from the mature protein)
Protein Source
Recombinant, host bacterium Bacillus subtilis WB800

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase Methanol 10% 100 100 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase Ethanol 10% 100 118 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase Isopropanol 10% 100 110 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase 1-Butanol 10% 100 120 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase Acetonitrile 10% 100 113 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase Acetone 10% 100 124 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase Methanol 30% 100 99 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase Ethanol 30% 100 97 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase Isopropanol 30% 100 92 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase 1-Butanol 30% 100 124 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase Acetonitrile 30% 100 60 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 410 nm) in aqueous phase Acetone 30% 100 120 % Incubation: 50 mM Na-phosphate buffer, Assay: 50 mM Tris– HCl, 5 mM CaCl2 Incubation: 8, Assay: 9 Incubation: 30Β°C, Assay: 65Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroanilide , Suc-Ala-Ala-Pro-Phe β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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