Lipase (gene metalip1) from an activated sludge metagenomic library
Enzyme Description
Species
Na (activated sludge metagenomic library)
Extremophile
No
but "Organic Solvent-Tolerant" enzyme
EC Number
Sequence
MNRIQVLLHAYQPFYEPLPKGSVDYRYFYRINALPLFHFQDLEIPMGVALERTTEVVVNLSVTRCDPAGILFNLGVRMSDHGGGWRGAVDSMILFRLNSGFEAAAVRVEKHSVPEMLMTPAILGADAALAWFTAHKGEIGLGAARISALVDVPRSAGGVSVGGNSCDYAENLAKQSSAAQILSVKTYELIIVQICDTHSYKEYVAYRFLTAGGMMKWFWRDYVCDGTREARVPASPLRTGTKLLDGTVLASAILEDDLRDEGESPLQRLDDQNHDLERAQWALSDGHYVCHWNAQSCHGTARNHVLDAFNNAHKLQQYANVGLVAHNPAW
Changhyun Roh et al. (2013)
β Isolation of an Organic Solvent-Tolerant Lipolytic Enzyme from Uncultivated Microorganism
Applied Biochemistry and Biotechnology
Β· doi:10.1007/s12010-013-0464-z β
Β· Activity - Classical
▼
Database ID
UniProt: D6R3X4 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (15 measurements)
15 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetone | 20% | 100 | 148 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | 1-Butanol | 20% | 100 | 185 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Chloroform | 20% | 100 | 138 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Cyclohexane | 20% | 100 | 185 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Diethyl Ether | 20% | 100 | 232 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100 | 258 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 210 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | 1,2-Dichloroethane | 20% | 100 | 165 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethyl Acetate | 20% | 100 | 135 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | n-Hexane | 20% | 100 | 130 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isooctane | 20% | 100 | 159 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dichloromethane | 20% | 100 | 130 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 20% | 100 | 195 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | 1-Propanol | 20% | 100 | 184 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Toluene | 20% | 100 | 140 | % | 20 mM Tris-HCl buffer | 7.5 | 28Β°C | 0.5 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) | Discrepancy in buffer concentration, also indicated as 50 mM |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.