Esterase (gene est-XG2) from an activated sludge sample metagenomic library

Enzyme Description

Species
Na (activated sludge sample metagenomic library)
Extremophile
No but "thermostable esterase"
EC Number
3.1.1.1 β†—

Sequence

Length: 501 amino acids
MGELVETRAGKLEGVREDGIRVFRGVPYAQPPVGGLRFRAPRPVEPWAGVRDAARFGPSAPQTELQIDFLPGMDVGAQSEDCLYLNVFAPGARGERRPVLVWIHGGAFTIGSGSQSLYDVRPIVRRGDVIVVTLNYRLGALGFLHLAESAGETFADAANAGVLDQVAALAWVRDNIAAFGGDPANVTIFGESAGGMSVGTLLGMPAARGLFQRAIAQSGAAHSANDAASAERVAAMFCEELGIAAGDGAALRRAPVEAILEAQARVGARTQSEQLLPFRPVVDGRALPEAPIEAVRRGLSREVAVIAGYTRDEWKLFVFMDPQGQKLDEPTLLARAEQRAPGRGEGLVRAYRAALGPAAKPYELFCALERDRIFGIPAVRLAEAQRRWQPNTYLYEFSWPAAMFGGLLGACHGIDVPFVMGSIGTPAGDRFAGAGAEALALQDRVMEAWLAFARSGDPRHAGLASWQPYDEGARLAMELGRRCEPLRLPDDALLRAWDGVL
Hua Shao et al. (2013) β€” Isolation and characterization of a thermostable esterase from a metagenomic library
Journal of Industrial Microbiology and Biotechnology  Β· doi:10.1007/s10295-013-1317-z β†—  Β· Stability - Incubation
10 measurements ▼
Database ID
UniParc: UPI0003845106 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 15% 100.0 103.14 % Incubation: ?, Assay: 50 mM Tris-HCl, 40% Ethanol, 2% acetonitrile Incubation: ?, Assay: 8.5 Incubation: Room temperture, Assay: 70Β°C 200 ΞΌM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 15% 100.0 82.36 % Incubation: ?, Assay: 50 mM Tris-HCl, 40% Ethanol, 2% acetonitrile Incubation: ?, Assay: 8.5 Incubation: Room temperture, Assay: 70Β°C 200 ΞΌM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 15% 100.0 102.25 % Incubation: ?, Assay: 50 mM Tris-HCl, 40% Ethanol, 2% acetonitrile Incubation: ?, Assay: 8.5 Incubation: Room temperture, Assay: 70Β°C 200 ΞΌM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 15% 100.0 84.36 % Incubation: ?, Assay: 50 mM Tris-HCl, 40% Ethanol, 2% acetonitrile Incubation: ?, Assay: 8.5 Incubation: Room temperture, Assay: 70Β°C 200 ΞΌM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 15% 100.0 94.41 % Incubation: ?, Assay: 50 mM Tris-HCl, 40% Ethanol, 2% acetonitrile Incubation: ?, Assay: 8.5 Incubation: Room temperture, Assay: 70Β°C 200 ΞΌM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 30% 100.0 62.3 % Incubation: ?, Assay: 50 mM Tris-HCl, 40% Ethanol, 2% acetonitrile Incubation: ?, Assay: 8.5 Incubation: Room temperture, Assay: 70Β°C 200 ΞΌM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 30% 100.0 68.54 % Incubation: ?, Assay: 50 mM Tris-HCl, 40% Ethanol, 2% acetonitrile Incubation: ?, Assay: 8.5 Incubation: Room temperture, Assay: 70Β°C 200 ΞΌM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 30% 100.0 67.68 % Incubation: ?, Assay: 50 mM Tris-HCl, 40% Ethanol, 2% acetonitrile Incubation: ?, Assay: 8.5 Incubation: Room temperture, Assay: 70Β°C 200 ΞΌM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 30% 100.0 62.58 % Incubation: ?, Assay: 50 mM Tris-HCl, 40% Ethanol, 2% acetonitrile Incubation: ?, Assay: 8.5 Incubation: Room temperture, Assay: 70Β°C 200 ΞΌM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30% 100.0 83.67 % Incubation: ?, Assay: 50 mM Tris-HCl, 40% Ethanol, 2% acetonitrile Incubation: ?, Assay: 8.5 Incubation: Room temperture, Assay: 70Β°C 200 ΞΌM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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