Proteolysin from Coprothermobacter proteolyticus

Enzyme Description

Species
Coprothermobacter proteolyticus β†—
Extremophile
Yes thermophilic organism
EC Number
3.4.21.66 β†—

Sequence

Length: 440 amino acids
MKKILLTLVIAVLLLSGFAGVKSAELLFVSNSTTTNQEDPENEIIVGYKENTDVAVLSKQVEKTTGAKLSRKGLKNFAVFKLPQGKAADVVMNQLKNDPNVEYVEPNYIAHAFDVPNDTFFNPYQWNFYDYGMTSNGYVSNYGIQAVSAWNITKGAGVKVAIIDTGVAYENYGAYTKAPDLANTLFDTANAYDFVNNDTHANDDNSHGTHVAGTIAQSTNNGMGAAGIAYQATILPIKVLDSEGSGTYDAIANGIIWAADKGARVINMSLGGSSGSTTLQNAIQYAYNKGVVIVCASGNDRRSTVSYPAAYTQCIAVGSTRFDGTRARYSNYGSALDIVAPGGDTSVDQNHDGYGDGILQQTFAEGSPTDFAYYFFQGTSMASPHVAGVAALVLSAHPTYTNEQVRTALQSTAKDLGTAGWDKYYGYGLVNAYAAVNWTP
Ana Toplak et al. (2013) β€” Proteolysin, a Novel Highly Thermostable and Cosolvent-Compatible Protease from the Thermophilic Bacterium Coprothermobacter proteolyticus
Applied and Environmental Microbiology  Β· doi:10.1128/AEM.01479-13 β†—  Β· Activity - Classical
18 measurements ▼
Database ID
UniProt: B5Y6G0 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli C43 (DE3)

Experimental Data (18 measurements)

18 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 40% 100.0 7.4 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Ethanol 60% 100.0 2.0 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Ethanol 50% 100.0 5.4 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Ethanol 40% 100.0 16.8 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Ethanol 30% 100.0 31.7 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Ethanol 20% 100.0 58.5 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Ethanol 10% 100.0 100.0 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 60% 100.0 1.5 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 50% 100.0 5.4 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100.0 100.0 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 30% 100.0 18.8 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 20% 100.0 41.1 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 10% 100.0 100.0 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 60% 100.0 0.5 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 50% 100.0 9.9 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 40% 100.0 28.8 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30% 100.0 56.5 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurment, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100.0 91.7 % 100 mM HEPES-NaOH buffer, 1 mM CaCl2, 10% organic solvent 7.5 40Β°C 1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide p-nitroaniline,N-succinyl-Ala-Ala-Pro-Phe β€” β€” Classical aqueous control (in %) | Due to substrate low solubility in water, the reference was taken as the assay with 10% of the given organic solvent

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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