(S)-specific carbonyl reductase (gene SCR2) from Candida parapsilosis

Enzyme Description

Species
Candida parapsilosis β†—
Extremophile
No
EC Number
1.1.1.184 β†—

Sequence

Length: 279 amino acids
MGEIESYCNKELGPLPTKAPTLSKNVLDLFSLKGKVASVTGSSGGIGWAVAEAYAQAGADVAIWYNSHPADEKAEHLQKTYGVRSKAYKCNISDPKSVEETISQQEKDFGTIDVFVANAGVPWTEGPEINVDNYDSWNKIINLDLNGVYYCAHTVGKIFKKNGKGSLVITSSMSGTIVNVPQLQAAYNAAKAACTHLTKSLAVEWAPFARVNCVSPGYIATEISDFVEKDMKAKWWQLTPLGREGLAQELVGAYLYLASNASTYTTGANLAVDGGYTCP
Zhong-Yu You et al. (2013) β€” Characterization of a newly synthesized carbonyl reductase and construction of a biocatalytic process for the synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate with high space-time yield
Applied Microbiology and Biotechnology  Β· doi:10.1007/s00253-013-5042-3 β†—  Β· Stability - Incubation
16 measurements ▼
Database ID
UniProt: D5G304 β†—
Sequence Annotation
Inferred
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethylformamide (DMF) 1% 100.0 93.0 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethylformamide (DMF) 5% 100.0 82.7 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 1% 100.0 92.3 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 5% 100.0 89.35 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase n-Hexane 50% 100.0 74.0 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Cyclohexane 50% 100.0 88.4 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase n-Heptane 50% 100.0 101.3 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Isooctane 50% 100.0 106.6 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase n-Dodecane 50% 100.0 93.4 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Ethyl Acetate 50% 100.0 22.2 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Butyl Acetate 50% 100.0 105.1 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Benzene 50% 100.0 84.8 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Toluene 50% 100.0 87.1 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Xylene 50% 100.0 86.0 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Phthalate 50% 100.0 131.5 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Petroleum Ether 50% 100.0 89.3 % Incubation: ?, Assay: 100 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 25Β°C, Assay: 45Β°C 2 mM Ethyl 4-chloroacetoacetate Ethyl (3S)-4-chloro-3-hydroxybutanoate 0.2 mM NADP β€” Non-incubated control (in %), activity measured in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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