Baeyer-Villiger monooxygenase (gene Af1) from Aspergillus fumigatus Af293
Enzyme Description
Sequence
MTRIRPDYDAIVIGAGFSGVRSLWELRRLGLTARCFDAGSDVGGTWWWNRYPGCRTDGEAWVYALKFLPELLEEWDFTERYPPQEEIQWYLSRVLDRYDLRKDIEFNTEVKSAHYSDHDSIWKITTASGKVATARYFLPATGITSIPKEPPFPGLQSFKGEVYQTSTWPAHEIEFENKRIGVIGTGSTGIQVITKLAPVAEQLIVFQRTPNYVIPAQNYPLDEKKREDIKKTFDATWDIAKRNLAGHAVKHSGRMVASAGGPEEIQRVFEDGWARGCYDFQLGTFDDSFMDPHANAVTSDFIRQKIRSIVRDGDTAEVLCPTYPFGARRPPCADGYYETFNRSNVKLVDIREDEIEVYDQGIKTASGAEYELDMIILALGFDTGTGAMNKIDIRGSQNRSLRESWTRRLETFAGVLVHGYPNMFVVCGPHLPAGNQPVSLEAFASWIGKTIEHMETNGLASIDVSNEAMDAWTTHVEQVWGGSFLAKHAHEQGSWFVGTNIPGKPSRIMFYFGGMVNLEPWLIKELETGWSSMSFTRL
Maria Laura Mascotti et al. (2013)
β Cloning, overexpression and biocatalytic exploration of a novel Baeyer-Villiger monooxygenase from Aspergillus fumigatus Af293
AMB Express
Β· doi:10.1186/2191-0855-3-33 β
Β· Stability - Incubation
▼
Database ID
UniProt: Q4WAZ0 β
Sequence Annotation
Inferred (only first 538 residues kept)
Protein Source
Recombinant, host bacterium Escherichia coli (TOP10)
Experimental Data (5 measurements)
5 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Methanol | 5% | 94.6 | 95.3 | % | Incubation: ?, Assay: 50 mM Tris-HCl | Incubation: ?, Assay: 8 | Incubation: Room temperature, Assay: 25Β°C | 2 mM Bicyclo(3.2.0)hept-2-en-6-one | (1R,5S)-3-Oxabicyclo[3.3.0]oct-6-en-2-one , regioisomeric lactones ((1S,5R)-2-oxabicyclo[3.3.0] oct-6-en-3-one | 100 ΞΌM NADP+ | β | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 5% | 94.6 | 89.5 | % | Incubation: ?, Assay: 50 mM Tris-HCl | Incubation: ?, Assay: 8 | Incubation: Room temperature, Assay: 25Β°C | 2 mM Bicyclo(3.2.0)hept-2-en-6-one | (1R,5S)-3-Oxabicyclo[3.3.0]oct-6-en-2-one , regioisomeric lactones ((1S,5R)-2-oxabicyclo[3.3.0] oct-6-en-3-one | 100 ΞΌM NADP+ | β | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | tert-Butanol | 5% | 94.6 | 95.7 | % | Incubation: ?, Assay: 50 mM Tris-HCl | Incubation: ?, Assay: 8 | Incubation: Room temperature, Assay: 25Β°C | 2 mM Bicyclo(3.2.0)hept-2-en-6-one | (1R,5S)-3-Oxabicyclo[3.3.0]oct-6-en-2-one , regioisomeric lactones ((1S,5R)-2-oxabicyclo[3.3.0] oct-6-en-3-one | 100 ΞΌM NADP+ | β | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | 1,4-Dioxane | 5% | 94.6 | 93.9 | % | Incubation: ?, Assay: 50 mM Tris-HCl | Incubation: ?, Assay: 8 | Incubation: Room temperature, Assay: 25Β°C | 2 mM Bicyclo(3.2.0)hept-2-en-6-one | (1R,5S)-3-Oxabicyclo[3.3.0]oct-6-en-2-one , regioisomeric lactones ((1S,5R)-2-oxabicyclo[3.3.0] oct-6-en-3-one | 100 ΞΌM NADP+ | β | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 5% | 94.6 | 99.3 | % | Incubation: ?, Assay: 50 mM Tris-HCl | Incubation: ?, Assay: 8 | Incubation: Room temperature, Assay: 25Β°C | 2 mM Bicyclo(3.2.0)hept-2-en-6-one | (1R,5S)-3-Oxabicyclo[3.3.0]oct-6-en-2-one , regioisomeric lactones ((1S,5R)-2-oxabicyclo[3.3.0] oct-6-en-3-one | 100 ΞΌM NADP+ | β | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.