Cutinase (gene Cut2) from Thermobifida fusca NRRL B-8184
Enzyme Description
Extremophile
Yes
"moderately thermophilic" organism
EC Number
Sequence
MAVMTPRRERSSLLSRALQVTAAAATALVTAVSLAAPAHAANPYERGPNPTDALLEASSGPFSVSEENVSRLSASGFGGGTIYYPRENNTYGAVAISPGYTGTEASIAWLGERIASHGFVVITIDTITTLDQPDSRAEQLNAALNHMINRASSTVRSRIDSSRLAVMGHSMGGGGTLRLASQRPDLKAAIPLTPWHLNKNWSSVTVPTLIIGADLDTIAPVATHAKPFYNSLPSSISKAYLELDGATHFAPNIPNKIIGKYSVAWLKRFVDNDTRYTQFLCPGPRDGLFGEVEEYRSTCPF
Krishnamoorthy Hegde et al. (2013)
β Production Optimization and Characterization of Recombinant Cutinases from Thermobifida fusca sp. NRRL B-8184
Applied Biochemistry and Biotechnology
Β· doi:10.1007/s12010-013-0219-x β
Β· Stability - Incubation
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Database ID
UniProt: Q6A0I4 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (16 measurements)
16 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Triethylamine | 40% | 100 | 76 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | 1-Heptanol | 40% | 100 | 62 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | 1-Hexanol | 40% | 100 | 14 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetone | 40% | 100 | 103 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 40% | 100 | 100 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Benzene | 40% | 100 | 23 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | 1-Butanol | 40% | 100 | 83 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dichloromethane | 40% | 100 | 39 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 40% | 100 | 114 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethylformamide (DMF) | 40% | 100 | 106 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Ethanol | 40% | 100 | 100 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Hexyl Acetate | 40% | 100 | 37 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 40% | 100 | 92 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | n-Hexane | 40% | 100 | 90 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Tetrahydrofuran (THF) | 40% | 100 | 101 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Triethylamine | 40% | 100 | 103 | % | Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 50Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.