Cutinase (gene Cut1) from Thermobifida fusca NRRL B-8184

Enzyme Description

Species
Thermobifida fusca NRRL B-8184 β†—
Extremophile
Yes "moderately thermophilic" organism
EC Number
3.1.1.74 β†—

Sequence

Length: 319 amino acids
MPPHAARPGPAQNRRGRAMAVITPRRERSSLLSRALRFTAAAATALVTAVSLAAPAHAANPYERGPNPTDALLEARSGPFSVSEERASRFGADGFGGGTIYYPRENNTYGAVAISPGYTGTQASVAWLGERIASHGFVVITIDTNTTLDQPDSRARQLNAALDYMINDASSAVRSRIDSSRLAVMGHSMGGGGTLRLASQRPDLKAAIPLTPWHLNKNWSSVRVPTLIIGADLDTIAPVLTHARPFYNSLPTSISKAYLELDGATHFAPNIPNKIIGKYSVAWLKRFVDNDTRYTQFLCPGPRDGLFGEVEEYRSTCPF
Krishnamoorthy Hegde et al. (2013) β€” Production Optimization and Characterization of Recombinant Cutinases from Thermobifida fusca sp. NRRL B-8184
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-013-0219-x β†—  Β· Stability - Incubation
14 measurements ▼
Database ID
UniProt: G8GER6 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Heptanol 40% 100 63 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Hexanol 40% 100 32 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 40% 100 100 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 40% 100 102 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Benzene 40% 100 21 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Butanol 40% 100 90 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dichloromethane 40% 100 26 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 40% 100 109 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethylformamide (DMF) 40% 100 110 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 40% 100 95 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Hexyl Acetate 40% 100 17 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 40% 100 98 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 40% 100 93 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Tetrahydrofuran (THF) 40% 100 100 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer, 4 % THF (Tetrahydrofuran), 10 mM sodium deoxycholate Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 50Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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