Laccase (gene cotA) from Bacillus licheniformis LS04

Enzyme Description

Species
Bacillus licheniformis LS04 β†—
Extremophile
No but "thermo-alkali-stable" enzyme
EC Number
1.10.3.2 β†—

Sequence

Length: 513 amino acids
MKLEKFVDRLPIPQVLQPQSKSKEMTYYEVTMKEFQQQLHRDLPPTRLFGYNGVYPGPTFEVQKHEKVAVKWLNKLPDRHFLPVDHTLHDDGHHEHEVKTVVHLHGGCTPADSDGYPEAWYTKDFHAKGPFFEREVYEYPNEQDATALWYHDHAMAITRLNVYAGLVGLYFIRDREERSLNLPKGEYEIPLLIQDKSFHEDGSLFYPRQPDNPSPDLPDPSIVPAFCGDTILVNGKVWPFAELEPRKYRFRILNASNTRIFELYFDHDITCHQIGTDGGLLQHPVKVNELVIAPAERCDIIVDFSRAEGKTVTLKNRIGCGGQDADPDTDADIMQFRISKPLKQKDTSSLPRILRKRPFYRRHKINALRNLSLGAAVDQYGRPVLLLNNTKWHEPVTETPALGSTEIWSIINAGRAIHPIHLHLVQFMILDHRPFDIERYQENGELVFTGPAVPPAPNEKGLKDTVKVPPGSVTRIIATFAPYSGRYVWHCHILEHEDYDMMRPLEVTDVRHQ
Lei Lu et al. (2013) β€” Cloning and expression of thermo-alkali-stable laccase of Bacillus licheniformis in Pichia pastoris and its characterization
Bioresource Technology  Β· doi:10.1016/j.biortech.2013.02.015 β†—  Β· Activity - Classical
20 measurements ▼
Database ID
UniProt: H8WGE4 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host yeast Pichia pastoris SMD1168H

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Acetone 30% 100.0 67.88 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 50% 100.0 0.0 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30% 100.0 8.13 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100.0 58.91 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100.0 118.0 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Acetonitrile 50% 100.0 46.64 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Acetonitrile 30% 100.0 70.48 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Acetonitrile 20% 100.0 96.55 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Acetonitrile 10% 100.0 112.99 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Acetone 50% 100.0 28.09 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Methanol 10% 100.0 142.58 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Acetone 20% 100.0 118.22 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Acetone 10% 100.0 132.57 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Ethanol 50% 100.0 24.25 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Ethanol 30% 100.0 85.01 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Ethanol 20% 100.0 121.36 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Ethanol 10% 100.0 131.75 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Methanol 50% 100.0 58.15 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Methanol 30% 100.0 105.37 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurment of the absorbance change by Syringaldazine oxidation, 525 nm) in the presence of organic solvent Methanol 20% 100.0 138.01 % 100 mM citrate-phosphate buffer 6 30Β°C 0.1 mM Syringaldazine Oxidized syrangaldazine β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*