Esterase rPPE01 from Pseudomonas putida ECU1011

Enzyme Description

Species
Pseudomonas putida ECU1011 β†—
Extremophile
No but organic-solvent tolerant enzyme
EC Number
3.1.1.6 β†—

Sequence

Length: 316 amino acids
GSPGVEQHTKAFLEALEQGGGKPLEQLSPKDARAVLTGAQASVKVDLSGIEVKERTIQANGQSIKLQVVRPANVKGELPVFMFFHGGGWVLGDFPTHQRLIRDLVVGSGAVAVYVDYTPSPESHYPTAINQAYAATQWVAEHGKEIGVDGKRLAVAGNSVGGNMAAVVALKAKEAGTPALRFQLLLWPVTDASFETASYKQFADGHFLTTGMMKWFWDNYTTDAKAREQIYASPLRASSEQLKGLPPALVQTAEFDVLRDEGEAYARKLNAAGVTVTSVRYNGMIHDYGLLNPLSQVPAVKAAMRQAGTELKVHLQ
Bao-Di Ma et al. (2013) β€” A thermostable and organic-solvent tolerant esterase from Pseudomonas putida ECU1011: Catalytic properties and performance in kinetic resolution of Ξ±-hydroxy acids
Bioresource Technology  Β· doi:10.1016/j.biortech.2013.01.089 β†—  Β· Stability - Incubation
24 measurements ▼
Database ID
UniProt: L7PYQ2 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (24 measurements)

24 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 50% 92 123 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (60 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Dibutyl Phthalate 50% 0 0 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Dibutyl Phthalate 50% 62 0 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dibutyl Phthalate 50% 92 0 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (60 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase n-Octane 50% 0 125 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase n-Octane 50% 62 133 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Octane 50% 92 145 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (60 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase n-Heptane 50% 0 160 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase n-Heptane 50% 62 169 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Heptane 50% 92 159 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (60 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase n-Hexane 50% 0 4 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase n-Hexane 50% 62 70 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase tert-Butyl Methyl Ether (MTBE) 50% 92 103 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (60 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Toluene 50% 0 0 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Toluene 50% 62 0 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Toluene 50% 92 68 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (60 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Butyl Acetate 50% 0 0 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Butyl Acetate 50% 62 0 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Butyl Acetate 50% 92 2 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
Stability - Incubation Activity after incubation (60 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Diisopropyl Ether 50% 0 115 % Incubation: ?, Assay: ? Incubation 100 mM PBS Incubation: ?, Assay: 7 Incubation: 30Β°C, Assay: ? 1 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 1000 rpm Water-incubated control (in %), activity measured in aqueous phase | Activity measured as a percentage of activity without incubaton
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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