Alcohol dehydrogenase (adh3 gene) from Oenococcus oeni
Enzyme Description
Sequence
MAERAYDFLMPSVNFFGPGVISKIGERAKMLGMKKPVIVTDKFLENLKNGAVAQTLASLKKSGVDYVVYNEVEPNPKIHNIKEVKTLYEKEDADSIITVGGGSAHDTGKGAGIIMTNGDDITKLAGIETLKNPLPPLIAVNTTAGTGSELTRHAVITNEETHLKFVVVSWRNIPLVSFNDPTLMLDIPKGLTAATGMDAFVQAVEPYVSVDHNPITDSQCIQAIKLIESSLREAVANGHNLQARTKMVEAEMLAGMAFNNANLGYVHAMAHQLGGQYDAPHGVCCALLLPYAEEYNLIADPERFAELARIMGENTDGLSTRDAAELSIKAMKQLSEDVGISHSIKDIGAKPEDFDLMAENALKDGNAFSNPRKGTKEDIVKIFQEAYDAK
Skander Elleuche et al. (2013)
β Structural and biochemical characterisation of a NAD+-dependent alcohol dehydrogenase from Oenococcus oeni as a new model molecule for industrial biotechnology applications
Applied Microbiology and Biotechnology
Β· doi:10.1007/s00253-013-4725-0 β
Β· Activity + Stability - Incubation
▼
Database ID
UniProt: U3UBS3 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli M15 (pREP4)
Experimental Data (1 measurement)
1 measurement
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurment, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50% | 100 | 0 | % | Incubation: 100 mM Tris-HCl, Assay: 100 mM Tris-HCl | Incubation: 9, Assay: 9 | Incubation: "On ice", Assay: 60Β°C | ? mM Ethanol | Acetaldehyde | 0.8 mM NAD+ | β | Correc control (in %), assay in organic solvent | Uncertainty about control |
Visualization : Activity + Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.