Copper-containing oxidase from Thermobifida fusca NTU10-1

Enzyme Description

Species

Thermobifida fusca NTU10-1 ↗

Extremophile

No but "moderately thermophilic" organism

EC Number

1.10.3.2 ↗ (UniProt annotations in contradiction with publication)

Sequence

Length: 241 amino acids

VTGTVVELAPGIHAGFTGRAGGVSGEPYATLNLGDHVGDDPAAVAENRRRAALGFGISPDRVVWMNQVHGATAVTVTGSGQAGDVDAVVTPEAGLALAVLVADCLPLLVADAAAGVIGAAHAGRPGMAAGVVPALVAEMARHGARPERCVALLGPAICGRCYEVPRDLQDRVARTVPEARCTTAEGTPGLDIRAGVTAQLTNLGVTNITHDSRCTRESADLFSYRRDATTGRFAGYVWRVP

Cheng-Yu Chen et al. (2013) — A 24.7-kDa copper-containing oxidase, secreted by Thermobifida fusca, significantly increasing the xylanase/cellulase-catalyzed hydrolysis of sugarcane bagasse

Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-4727-y ↗ · Activity - Classical

16 measurements ▼

Database ID

UniProt: Q47QW5 ↗

Sequence Annotation

Explicit - Provided (first residue from UniProt sequence replace by V)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Methanol	10%	100	79	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Methanol	20%	100	69	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Ethanol	10%	100	99	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Ethanol	20%	100	85	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Isopropanol	10%	100	79	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Isopropanol	20%	100	38	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Acetone	10%	100	89	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Acetone	20%	100	29	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Acetonitrile	10%	100	69	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Acetonitrile	20%	100	2	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	59	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	25	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	16	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	0	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Formaldehyde	3.7%	100	66	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Formaldehyde	7.4%	100	1	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Methanol	10%	100	79	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Methanol	20%	100	69	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Ethanol	10%	100	99	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Ethanol	20%	100	85	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Isopropanol	10%	100	79	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Isopropanol	20%	100	38	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Acetone	10%	100	89	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Acetone	20%	100	29	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Acetonitrile	10%	100	69	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Acetonitrile	20%	100	2	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	59	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	25	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	16	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	0	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Formaldehyde	3.7%	100	66	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (measurment of the absorbance change by 2.6-DMP oxidation, 470 nm) in the presence of organic solvent	Formaldehyde	7.4%	100	1	%	20 mM phosphate buffer	8	50°C	2 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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