Ξ²-galactosidase (gene bga) from Halorubrum lacusprofundi
Enzyme Description
Species
Extremophile
Yes
"halophilic" and "psychrophilic" organism
EC Number
Sequence
MRLGVCYFPEHWPSEEWERDVAAMADAGLEYVRMAEFSWGVLEPERGTFDFEWLDEAIELIGDHGMQAVLCTPTATPPKWLVDERPSIRQEDPDGTVREHGSRRHYCFNSDAYREETARIVERVTERYADSPHVAGWQTDNEFGCHETVRCYCDDCADAFRTWLADRYGDIDRLNEAWGNAFWSQQYGSFDEIDPPGPTPAEHHPSRLLAYARFSSDSVVEYNRLHADLIREADPDWFVTHNFMGRFPTLNAYDVSEDLDRVAWDSYPTGFVQDRYDGEASPDQLRAGDPDQVGMDHDIYRSALDRPFWVMEQQPGDVNWPPHCPQPGEGAMRLWAHHAAAHGADAVLYFRWRRCLEGQEQYHAGLRKADGSPDRGYADAAHTSEEFATLDGASHVDAPVAVVFDYDSLWALNAQPHAPDFDYWALQEAFYGAVRGRGVQVDVVPPSADLSGYAAVVAPALHLVTEDLADRLTDYIAGGGEVLFGPRTGVKDAENKLRPMSQPGPLTDLVGATVDQHESLPRRLETTVRRVGDPTDDSEEIAAPPVSFRTWAEWLDPDAAEPQYAYDVDGPADGRPAVVTNTVGDGQVTYCGVWPESDLADALASDLLDRAGVRYAERLPDGVRIGYRGGRTWVTNFTSDRLRLPEIDPESLAVDDTDRDGFDPMADDDKDSSADGIVVGPYGVAVIEGDCVDGLRIAQT
Ram Karan et al. (2013)
β Cloning, overexpression, purification, and characterization of a polyextremophilic Ξ²-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi
BMC Biotechnology
Β· doi:10.1186/1472-6750-13-3 β
Β· Stability - Incubation
▼
Database ID
UniProt: B9LW38 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host archaeon Halobacterium sp. NRC-1
Experimental Data (8 measurements)
8 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 5% | 100 | 105 | % | ? | Incubation: ?, Assay: 6.5 | Incubation: 30Β°C, Assay: 25Β°C | 2.2 mM O-nitrophenyl-Ξ²-D-galactopyranoside | galactose , o-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 10% | 100 | 90 | % | ? | Incubation: ?, Assay: 6.5 | Incubation: 30Β°C, Assay: 25Β°C | 2.2 mM O-nitrophenyl-Ξ²-D-galactopyranoside | galactose , o-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Ethanol | 5% | 100 | 70 | % | ? | Incubation: ?, Assay: 6.5 | Incubation: 30Β°C, Assay: 25Β°C | 2.2 mM O-nitrophenyl-Ξ²-D-galactopyranoside | galactose , o-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Ethanol | 10% | 100 | 25 | % | ? | Incubation: ?, Assay: 6.5 | Incubation: 30Β°C, Assay: 25Β°C | 2.2 mM O-nitrophenyl-Ξ²-D-galactopyranoside | galactose , o-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Butanol | 5% | 100 | 73 | % | ? | Incubation: ?, Assay: 6.5 | Incubation: 30Β°C, Assay: 25Β°C | 2.2 mM O-nitrophenyl-Ξ²-D-galactopyranoside | galactose , o-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Butanol | 10% | 100 | 33 | % | ? | Incubation: ?, Assay: 6.5 | Incubation: 30Β°C, Assay: 25Β°C | 2.2 mM O-nitrophenyl-Ξ²-D-galactopyranoside | galactose , o-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Isoamyl Alcohol | 5% | 100 | 79 | % | ? | Incubation: ?, Assay: 6.5 | Incubation: 30Β°C, Assay: 25Β°C | 2.2 mM O-nitrophenyl-Ξ²-D-galactopyranoside | galactose , o-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Isoamyl Alcohol | 10% | 100 | 30 | % | ? | Incubation: ?, Assay: 6.5 | Incubation: 30Β°C, Assay: 25Β°C | 2.2 mM O-nitrophenyl-Ξ²-D-galactopyranoside | galactose , o-Nitrophenol | β | Incubation: 200 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
Visualization : Stability β Incubation
Ram Karan et al. (2013)
One bar per measurement. Colour = solvent, shade = solvent volume.
Ram Karan et al. (2020)
β Understanding High-Salt and Cold Adaptation of a Polyextremophilic Enzyme
Microorganisms
Β· doi:10.3390/microorganisms8101594 β
Β· Stability - Incubation
▼
Database ID
UniProt: B9LW38 β
Sequence Annotation
Explicit - Provided PDB Accession Number
Protein Source
Recombinant, host archaeon Haloferax volcanii H1895
Experimental Data (20 measurements)
20 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Butanol | 20% | 100 | 78 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 73 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 90 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 83 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 93 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Pentanol | 20% | 100 | 63 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Pentanol | 20% | 100 | 78 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Pentanol | 20% | 100 | 89 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Pentanol | 20% | 100 | 97 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Butanol | 20% | 100 | 60 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 20% | 100 | 81 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Butanol | 20% | 100 | 84 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Butanol | 20% | 100 | 93 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Ethanol | 20% | 100 | 64 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Ethanol | 20% | 100 | 70 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Ethanol | 20% | 100 | 75 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Ethanol | 20% | 100 | 82 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 20% | 100 | 55 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 20% | 100 | 66 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 20% | 100 | 75 | % | Incubation: ? Buffer, 2 M NaCl, Assay: ? | Incubation: ?, Assay: 6.5 | Incubation: Room temperature, 50Β°C assay | 2.2 mM o-Nitrophenyl-Ξ²-D-galactopyranosidee | D-Galactose , o-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
Visualization : Stability β Incubation
Ram Karan et al. (2020)
One bar per measurement. Colour = solvent, shade = solvent volume.