Fumarate hydratase from Sulfolobus solfataricus

Enzyme Description

Species

Sulfolobus solfataricus ↗

Extremophile

Yes "thermophilic" organism

EC Number

4.2.1.2 ↗

Sequence

Length: 438 amino acids

MKYTDTAPKLFMNTGTKFPRRIIWSMGVLKKSCAKVNADLGLLDKKIADSIIKASDDLIDGKLDDKIVLDVFQTGSGTGLNMNVNEVIAEVASSYSNLKVHPNDHVNFGQSSNDTVPTAIRIAAVAEVTNRLLPALQQIISSLNKKAEEYKDVIKAGRTHLRDALPVTLGQELSAYADAFQHEHEQVMNILEYVKELPIGGTATGTGLNSHPEFQERVINEINRITGLGFKPANRFRAMRLLTDLLLLSGALRNIAVDLYRLGQDIRLMFSGPLTGLNEIDLPTQEEIAGSSIMPGKTNPVTVEATLLISAQVVGLDHANQFASMLGEFELSMGIPLVGYNIVTQVNFISEALEKMSRLVIDGMVANVEKMKRYAESSPSLITIVSPVIGYDKATEIGKKLNKGMSIREALRELGYSDNEINKILDLSKLVKPGFTAK

S. Puchegger et al. (1990) — Purification and properties of a thermostable fumarate hydratase from the archaeobacterium Sulfolobus solfataricus

Journal of General Microbiology · doi:10.1099/00221287-136-8-1537 ↗ · Activity - Classical Stability - Incubation

9 measurements ▼

Database ID

UniProt: P39461 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, archaeon Sulfolobus solfataricus

Experimental Data (9 measurements)

9 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in the presence of organic solvent	Methanol	40%	100	94	%	100 mM potassium buffer	7.5	70°C	50 mM L-Malic acid	Fumarate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in the presence of organic solvent	Isopropanol	40%	100	18	%	100 mM potassium buffer	7.5	70°C	50 mM L-Malic acid	Fumarate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in the presence of organic solvent	Ethanol	40%	100	18	%	100 mM potassium buffer	7.5	70°C	50 mM L-Malic acid	Fumarate	—	—	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (10 hours at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Ethanol	50%	100	86	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (10 hours at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Methanol	50%	100	102	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (10 hours at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Isopropanol	50%	100	96	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (14 days at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Ethanol	50%	100	83	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (14 days at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Methanol	50%	100	104	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (14 days at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Isopropanol	50%	100	85	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase

Activity - Classical	Activity measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in the presence of organic solvent	Methanol	40%	100	94	%	100 mM potassium buffer	7.5	70°C	50 mM L-Malic acid	Fumarate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in the presence of organic solvent	Isopropanol	40%	100	18	%	100 mM potassium buffer	7.5	70°C	50 mM L-Malic acid	Fumarate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in the presence of organic solvent	Ethanol	40%	100	18	%	100 mM potassium buffer	7.5	70°C	50 mM L-Malic acid	Fumarate	—	—	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (10 hours at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Ethanol	50%	100	86	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (10 hours at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Methanol	50%	100	102	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (10 hours at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Isopropanol	50%	100	96	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (14 days at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Ethanol	50%	100	83	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (14 days at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Methanol	50%	100	104	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase
Stability - Incubation	Activity after incubation (14 days at 20°C), measured by absorbance spectrophotometry (fumarate absorbance measurement, 240 nm) in aqueous phase	Isopropanol	50%	100	85	%	Incubation: 100 mM potassium buffer, Assay: 100 mM potassium buffer	7.5 incubation, 7.5 buffer	Incubation: 20°C, Assay: 70°C	50 mM L-Malic acid	Fumarate	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation