Laccase Ssl1 from Streptomyces sviceus

Enzyme Description

Species

Extremophile

EC Number

1.10.3.2 ↗

Sequence

Length: 325 amino acids

MGALDRRGFNRRVLLGGAAVATSLSLAPEARSDAGPAQAAPGGEVRRIKLYAERLADGQMGYGLEKGRATIPGPLIELNEGDTLHIEFENTMDVRASLHVHGLDYEVSSDGTTLNKSDVEPGGTRTYTWRTHAPGRRSDGTWRAGSAGYWHYHDHVVGTEHGTGGIRKGLYGPVIVRRKGDVLPDATHTIVFNDMLINNRPAHSGPNFEATVGDRVEFVMITHGEYYHTFHMHGHRWADNRTGMLTGPDDPSQVVDNKIVGPADSFGFQVIAGEGVGAGAWMYHCHVQSHSDMGMVGLFLVKKTDGTIPGYEPHEHSGQRAEHHH

Matthias Gunne et al. (2012) — Characterization of the Alkaline Laccase Ssl1 from Streptomyces sviceus with Unusual Properties Discovered by Genome Mining

PLoS ONE · doi:10.1371/journal.pone.0052360 ↗ · Activity - Classical Stability - Incubation

16 measurements ▼

Database ID

UniProt: B5HSR1 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	n-Hexane	50%	100	101	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	Assay: 600 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Isooctane	50%	100	99	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	Assay: 600 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	21	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Methanol	40%	100	40	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Ethanol	40%	100	33	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Isopropanol	40%	100	23	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Acetonitrile	40%	100	24	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Acetone	40%	100	21	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	n-Hexane	50%	73	73	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	Incubation: 600 rpm	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Isooctane	50%	73	74	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	Incubation: 600 rpm	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	73	132	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Methanol	40%	73	75	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Ethanol	40%	73	71	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Isopropanol	40%	73	79	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Acetonitrile	40%	73	53	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Acetone	40%	73	86	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent

Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	n-Hexane	50%	100	101	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	Assay: 600 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Isooctane	50%	100	99	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	Assay: 600 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	21	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Methanol	40%	100	40	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Ethanol	40%	100	33	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Isopropanol	40%	100	23	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Acetonitrile	40%	100	24	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (2.6-DMP absorbance measurement, 468 nm) in the presence of organic solvent	Acetone	40%	100	21	%	50 mM glycine-sodium hydroxide buffer	9	Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Classical aqueous control (in %)
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	n-Hexane	50%	73	73	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	Incubation: 600 rpm	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Isooctane	50%	73	74	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	Incubation: 600 rpm	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	73	132	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Methanol	40%	73	75	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Ethanol	40%	73	71	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Isopropanol	40%	73	79	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Acetonitrile	40%	73	53	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent
Stability - Incubation	Residual activity after incubation (20 hours at room temperature), measured by absorbance spectrophotometry (2.6-DMP absorbance, measurement 468 nm) in aqueous phase	Acetone	40%	73	86	%	Incubation: 50 mM glycine-sodium hydroxide buffer, Assay: 50 mM glycine-sodium hydroxide buffer	Incubation: 9, Assay: 9	Incubation: Room temperature, Assay: Room temperature	0.5 mM 2,6-Dimethoxyphenol	2,6-dimethoxyphenoxyl radical reaction product	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether activity was measured with or without organic solvent

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation