Esterase (gene Est0796) from Lactobacillus plantarum WCFS1

Enzyme Description

Species
Lactobacillus plantarum WCFS1 β†—
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 249 amino acids
MMLKQPEPFFFEHGQHAVILLHAYAGSANDVRMLARALEREDYTVYGPQFSGHATDDPRDILAQTPAQWWQDTQQAISFMRQKGYTKISIFGLSLGGIFATAALERDPQLLGGGTFSSPLFAGNRSDVAEMFITLSHHQLAHSQFSIAEREQILMTLPELVQRQLQAVNTFTTTEVTSHLSAVTQPFFIGQGGQDELIDATVARQLRDQLPQVPVDFHWYADAGHVITVNSAHHQLEQDVLTYLKTIYK
Navarro-GonzΓ‘lez Inmaculada et al. (2012) β€” Overexpression, Purification, and Biochemical Characterization of the Esterase Est0796 from Lactobacillus plantarum WCFS1
Molecular Biotechnology  Β· doi:10.1007/s12033-012-9607-7 β†—  Β· Stability - Incubation
12 measurements ▼
Database ID
UniProt: F9UM18 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli Rosetta (DE3)

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 5% 100 60 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 5% 100 79 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 5% 100 47 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 5% 100 68 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 5% 100 70 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Propanol 5% 100 76 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 10% 100 59 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 10% 100 72 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 10% 100 38 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 10% 100 17 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 62 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Propanol 10% 100 64 % Incubation: 100 mM potassium phosphate buffer, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 25Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in aqueous phase | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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