Cytochrome P450 3A5 from Homo sapiens

Enzyme Description

Species
Homo sapiens (human) β†—
Extremophile
No
EC Number
1.14.14.1 β†—

Sequence

Length: 502 amino acids
MDLIPNLAVETWLLLAVSLVLLYLYGTRTHGLFKRLGIPGPTPLPLLGNVLSYRQGLWKFDTECYKKYGKMWGTYEGQLPVLAITDPDVIRTVLVKECYSVFTNRRSLGPVGFMKSAISLAEDEEWKRIRSLLSPTFTSGKLKEMFPIIAQYGDVLVRNLRREAEKGKPVTLKDIFGAYSMDVITGTSFGVNIDSLNNPQDPFVESTKKFLKFGFLDPLFLSIILFPFLTPVFEALNVSLFPKDTINFLSKSVNRMKKSRLNDKQKHRLDFLQLMIDSQNSKETESHKALSDLELAAQSIIFIFAGYETTSSVLSFTLYELATHPDVQQKLQKEIDAVLPNKAPPTYDAVVQMEYLDMVVNETLRLFPVAIRLERTCKKDVEINGVFIPKGSMVVIPTYALHHDPKYWTEPEEFRPERFSKKKDSIDPYIYTPFGTGPRNCIGMRFALMNMKLALIRVLQNFSFKPCKETQIPLKLDTQGLLQPEKPIVLKVDSRDGTLSGE
Vanessa GonzΓ‘lez-PΓ©rez et al. (2012) β€” Impact of Organic Solvents on Cytochrome P450 Probe Reactions: Filling the Gap with (S)-Warfarin and Midazolam Hydroxylation
Drug Metabolism and Disposition  Β· doi:10.1124/dmd.112.047134 β†—  Β· Activity - Classical
18 measurements ▼
Database ID
UniProt: P20815 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host insect cells

Experimental Data (18 measurements)

18 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 0.1% 100 93 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Isopropanol 2% 100 83 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Isopropanol 1% 100 101 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Isopropanol 0.1% 100 109 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Acetone 2% 100 47 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Acetone 1% 100 61 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Acetone 0.1% 100 100 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 2% 100 48 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 1% 100 58 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Acetonitrile 0.1% 100 122 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Ethanol 2% 100 113 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Ethanol 1% 100 108 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Ethanol 0.1% 100 110 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Methanol 2% 100 96 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Methanol 1% 100 96 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Methanol 0.1% 100 116 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Acetonitrile 2% 100 108 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC-coupled mass spectrometry in the presence of organic solvent Acetonitrile 1% 100 122 % 100 mM potassium phosphate buffer 7.4 37Β°C 4 Β΅M Midazolam 1β€²-Hydroxymidazolam 1 mM NADPH β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume. β€” β€” β€” Reference value. Hover for details.

Structure

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