Lipase from Pseudomonas sp. TK-3
Enzyme Description
Species
Extremophile
No
but "psychrotrophic" organism and "cold-adapted " enzyme
EC Number
Sequence
MGIFDYKNLGTEGSKALFADAMAITLYTYHNLDNGFAVGYQHNGLGLGLPATLVGALLGSTDSQGVIPGIPWNPDSEKAALDAVQKAGWTPISASALGYGGKVDARGTFFGEKAGYTTAQVEVLGKYDDAGKLLEIGIGFRGTSGPRETLISDSIGDLVSDLLAALGPKDYAKNYAGEAFGGLLKNVADYASAHGLSGKDVVVSGHSLGGLAVNSMADLSNSKWSGFYKDANYVAYASPTQSAGDKVLNIGYENDPVFRALDGSSFNLSSLGVHDKPHESTTDNIVSFNDHYASTLWNVMPFSIVNLPTWISHLPTGYGDGMTRILESGFYEQMTRDSTVIVANLSDPARANTWVQDLNRNAEPHKGNTFIIGSDGNDLIQGGKGADFIEGGKGNDTIRDNSGHNTFLFSGHFGTDRVIGYQTTDKLLFKDVEGSTDLRDHAKVVGADTVLTFGADSVTLVGVGHGGLWADGVSIG
Daisuke Tanaka et al. (2012)
β Characterization of a New Cold-adapted Lipase from Pseudomonas sp. TK-3
Applied Biochemistry and Biotechnology
Β· doi:10.1007/s12010-012-9776-7 β
Β· Activity + Stability - Incubation
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Database ID
UniProt: I0IJN6 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (4 measurements)
4 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 59 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic, 5 mM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 1.25 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 20% | 100 | 38 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic, 5 mM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 1.25 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 20% | 100 | 88 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic, 5 mM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 1.25 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isopropanol | 20% | 100 | 59 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.4% Triton X-100, 0.1% gum arabic, 5 mM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 1.25 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
Visualization : Activity + Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.