Lipase SMlipA from a soil metagenomic library

Enzyme Description

Species
Na (soil metagenomic library)
Extremophile
No but "organic-solvent stable" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 429 amino acids
EASKTLFADATAITLYTYHNLDNGFAVGYQQRGLGLGLPATLVGALLGSTDSQGVIMRTPLSAAEFATSVAPLYLLELSFDSGALRPSGVFKKFILEAEDTVIVASAGKLNLVGIGFRGTSGPRDTLIDSLVDLVSVDLLAALGPKDYAKNYAGEAFGGLLKTVADYAGAHGLSGKDVLVSGHSLGGLAVNSMADLSTSKWAGFYKDTLYAAALWKQQAKGDTSLPGGYTLGSDPFLTQLDAEAFLPQEQKDALVHDKAHESTTDNIVSFNDHYASTLWNVLPFSIANLSTWVSHLPSAYGDGMTRVLESGFYEQMTRDSTIILCPTWSDPARANTWVQDLNRNAEPHTGNTFIIGSDGNDLIQGGKGADFIEGGKGNDTIRDNSGHNTFLFSGHFGQDRIIGYQPTGCSRAPTAAPTCATTRRPGPIR
Mahejibin Khan et al. (2012) β€” Expression and purification of organic solvent stable lipase from soil metagenomic library
World Journal of Microbiology and Biotechnology  Β· doi:10.1007/s11274-012-1051-0 β†—  Β· Activity + Stability - Incubation
10 measurements ▼
Database ID
UniParc: UPI000243DBC1 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli JM109

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Activity after incubation (2.5 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isopropanol 50% 100.0 182.65 % ? Incubation: ?, Assay: 7.5 Incubation: 37Β°C, Assay: 30Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (2.5 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 50% 100.0 144.07 % ? Incubation: ?, Assay: 7.5 Incubation: 37Β°C, Assay: 30Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (2.5 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 50% 100.0 157.61 % ? Incubation: ?, Assay: 7.5 Incubation: 37Β°C, Assay: 30Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (2.5 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Xylene 50% 100.0 230.87 % ? Incubation: ?, Assay: 7.5 Incubation: 37Β°C, Assay: 30Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (2.5 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent n-Hexane 50% 100.0 205.53 % ? Incubation: ?, Assay: 7.5 Incubation: 37Β°C, Assay: 30Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isopropanol 50% 100.0 185.02 % ? Incubation: ?, Assay: 7.5 Incubation: 37Β°C, Assay: 30Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 50% 100.0 142.46 % ? Incubation: ?, Assay: 7.5 Incubation: 37Β°C, Assay: 30Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 50% 100.0 165.39 % ? Incubation: ?, Assay: 7.5 Incubation: 37Β°C, Assay: 30Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Xylene 50% 100.0 235.57 % ? Incubation: ?, Assay: 7.5 Incubation: 37Β°C, Assay: 30Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent n-Hexane 50% 100.0 201.4 % ? Incubation: ?, Assay: 7.5 Incubation: 37Β°C, Assay: 30Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Visualization : Activity + Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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