Ξ±2,3-sialyltransferase from Photobacterium sp. JT-ISH-224

Enzyme Description

Species
Photobacterium sp. JT-ISH-224 β†—
Extremophile
No
EC Number
2.4.99.6 β†—

Sequence

Length: 409 amino acids
MLVFCKKMFFSVFISLMILGGCNSDSNHNNSDGNITKNKTIEVYVDRATLPTIQQMTQIINENSNNKKLISWSRYPINDEELLESINGSFFKNNSELIKSLDSMILTNDIKKVIINGNTLWAADVVNIIKSIEAFGKKTEIELNFYDDGSAEYVRLYDFSKLPESEQEYKISLSKDNILSSINGTQPFENVVENIYGFSQLYPTTYHMLRADIFETNLPLRSLKGVLSNNIKQMKWDYFKTFNSQQKDKFYNFTGFNPDEIMEQYKASPNKNFIFVGTNSGTATAEQQIDILTEAKNPNSPIITKSIQGFDLFFKGHPSATYNKQIIDAHNMIEIYNKIPFEALIMTDALPDAVGGMGSSVFFSLPNTVENKFIFYKSDTDIENNALIQVMIELNIVNRNDVKLISDLQ
Izuru Nagashima et al. (2012) β€” Efficiency of organic solvents on the ability of Ξ±2,3-sialyltransferase from Photobacterium sp. JT-ISH-224 to control a hydrolysis side reaction
Carbohydrate Research  Β· doi:10.1016/j.carres.2012.06.007 β†—  Β· Activity + Stability - Conversion
20 measurements ▼
Database ID
UniProt: A8QYL0 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Methanol 20% 49 42 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Acetone 25% 49 56 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Acetone 20% 49 60 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Acetone 15% 49 48 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Acetone 10% 49 46 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Acetonitrile 25% 49 0 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Acetonitrile 20% 49 6 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Acetonitrile 15% 49 33 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Acetonitrile 10% 49 74 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Methanol 25% 49 46 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Dimethyl Sulfoxide (DMSO) 10% 49 32 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Methanol 15% 49 35 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Methanol 10% 49 44 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Ethanol 25% 49 20 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Ethanol 20% 49 50 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Ethanol 15% 49 53 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Ethanol 10% 49 47 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Dimethyl Sulfoxide (DMSO) 25% 49 16 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Dimethyl Sulfoxide (DMSO) 20% 49 20 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)
Activity + Stability - Conversion Conversion after incubation (2 hours at 30Β°C), measured by HPLC (note : reaction is in competition with hydrolysis reaction) Dimethyl Sulfoxide (DMSO) 15% 49 25 % 100 mM Bis–Tris 6 30Β°C 0.5 mM 4-metylumbelliferyl lactoside, 2.5 mM cytidine-50 -monophospho-b-D- N-acetylneuraminic acid, disodium salt cytidine monophosphate , Ξ±2,3-sialylated 4-methylumbelliferyl lactoside β€” β€” Classical aqueous control (in %)

Visualization : Activity + Stability β€” Conversion

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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