Esterase from Psychrobacter cryohalolentis K5T
Enzyme Description
Extremophile
Yes
"psychrophilic" organism
EC Number
Sequence
MLLKRLGLATLLSFSVVGCTTAPNTLAINTTQKIIQYERSKSDLTTQSFTLSSGDKIVYAENGNVAGEPLLLVHGFGGNKDNFTRIARQLENYNLIIPDLLGFGDSSKPMAADYHSEAQATRLHELLQAKGLASSIHVGGNSMGGAISVAYAAKYPKEVKSLWLIDSAGFWSAGVPKSLESATLENNPLLVDKKEDFYAMYDFVMSKPPYIPKSVKAVFAQERIANKALESKILAQIVEDNVEQRAKVITEYNIPTLVVWGEEDKVIKPETVTLIKEIIPQSQVITMPKIGHVPMIEAVKDTANDYKAFREGLKN
Ksenia Novototskaya-Vlasova et al. (2012)
β Cloning, purification, and characterization of a cold-adapted esterase produced by Psychrobacter cryohalolentis K5T from Siberian cryopeg
FEMS Microbiology Ecology
Β· doi:10.1111/j.1574-6941.2012.01385.x β
Β· Activity + Stability - Incubation
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Database ID
UniProt: Q1QEU6 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (10 measurements)
10 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm) in organic solvent | Acetonitrile | 5% | 100.0 | 28.0 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.5% (w/v) Triton X-100, 0.1 M NaCl | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 0.25 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm) in organic solvent | Ethanol | 5% | 100.0 | 88.3 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.5% (w/v) Triton X-100, 0.1 M NaCl | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 0.25 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm) in organic solvent | Methanol | 5% | 100.0 | 92.0 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.5% (w/v) Triton X-100, 0.1 M NaCl | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 0.25 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm) in organic solvent | Dimethyl Sulfoxide (DMSO) | 5% | 100.0 | 105.4 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.5% (w/v) Triton X-100, 0.1 M NaCl | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 0.25 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm) in organic solvent | Dimethylformamide (DMF) | 5% | 100.0 | 45.7 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.5% (w/v) Triton X-100, 0.1 M NaCl | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 0.25 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm) in organic solvent | Acetonitrile | 10% | 100.0 | 1.6 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.5% (w/v) Triton X-100, 0.1 M NaCl | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 0.25 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm) in organic solvent | Ethanol | 10% | 100.0 | 60.0 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.5% (w/v) Triton X-100, 0.1 M NaCl | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 0.25 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm) in organic solvent | Methanol | 10% | 100.0 | 69.0 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.5% (w/v) Triton X-100, 0.1 M NaCl | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 0.25 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm) in organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 95.0 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.5% (w/v) Triton X-100, 0.1 M NaCl | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 0.25 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 415 nm) in organic solvent | Dimethylformamide (DMF) | 10% | 100.0 | 9.0 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.5% (w/v) Triton X-100, 0.1 M NaCl | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 25Β°C | 0.25 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), reaction in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
Visualization : Activity + Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.