Endo-β-1,4-mannanase from Bacillus subtilis G1

Enzyme Description

Species

Extremophile

EC Number

3.2.1.78 ↗

Sequence

Length: 364 amino acids

MGSCIVKNIRSLAHYILLRLLFSKTNEAHTVSPVNPNAQQTTKAVMNWLAHLPNRTENRVLSGAFGGYSHDTFSMAEADRIRSATGQSPAIYGCDYARGWLETAKIEDSIDVSCNGDLISYWKNGGIPQISLHLANPAFQSGHFKTPITNDQYKKILDSSTAEGKRLNAMLSKIADGLQELENQGVPVLFRPLHEMNGEWFWWGLTSYNQKDNERISLYKQLYKKIYHYMTDTRGLDHLIWVYSPDANRDFKTDFYPGASYVDIVGLDAYFQDAYSINGYDQLTALNKPFAFTEVGPQPANGSFDYSLFINAIKQKYPKTIYFLAWNDEWSPAVNKGASALYHDSWTLNKGEIWNGDSLTPIVE

Thi Thu Hang Vu et al. (2012) — Cloning, High-Level Expression, Purification, and Properties of a Novel Endo-β-1,4-Mannanase from Bacillus subtilis G1 in Pichia pastoris

Journal of Microbiology and Biotechnology · doi:10.4014/jmb.1106.06052 ↗ · Stability - Incubation

15 measurements ▼

Database ID

UniProt: Q2LGZ6 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host yeast Pichia pastoris GS115

Experimental Data (15 measurements)

15 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Methanol	10%	100	78	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Ethanol	10%	100	89	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Isopropanol	10%	100	100	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	1-Butanol	10%	100	65	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Acetone	10%	100	85	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Methanol	20%	100	20	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Ethanol	20%	100	71	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Isopropanol	20%	100	106	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	1-Butanol	20%	100	1	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Acetone	20%	100	88	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Methanol	30%	100	3	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Ethanol	30%	100	88	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Isopropanol	30%	100	103	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	1-Butanol	30%	100	0	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS method)	Acetone	30%	100	46	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent

Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Methanol	10%	100	78	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Ethanol	10%	100	89	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Isopropanol	10%	100	100	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	1-Butanol	10%	100	65	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Acetone	10%	100	85	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Methanol	20%	100	20	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Ethanol	20%	100	71	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Isopropanol	20%	100	106	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	1-Butanol	20%	100	1	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Acetone	20%	100	88	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Methanol	30%	100	3	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Ethanol	30%	100	88	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	Isopropanol	30%	100	103	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS assay) in aqueous phase	1-Butanol	30%	100	0	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (24 hours at 37°C), measured by absorbance spectrophotometry (DNS method)	Acetone	30%	100	46	%	Incubation: ?, Assay: 20 mM potassium buffer	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 40°C	0.5% (w/v) Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), reaction in aqueous phase \| Uncertainty about whether assay done with or without organic solvent

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate · Scroll to zoom · Right-click drag to pan · Powered by Mol*