Esterase (gene EstC) from Streptomyces coelicolor A3(2)

Enzyme Description

Species
Streptomyces coelicolor A3(2) β†—
Extremophile
No but "cold-active" enzyme
EC Number
3.1.1.1 β†—

Sequence

Length: 298 amino acids
MSRNAAFSPPPSARAYRLRTARGEFAVVDSPAAAGVAPRGVVMMLPGFTGSKEDFTLLHRPLGARGYRTVAVDGRGQYESDGPEHDESAYARPELARDVLAQAAALGTRVHLVGHSLGGQIARAAVLLDPAPFLSLTLMSSGPARISESQRQRVKLLRDALDTMTMAEVWEVMQAMGPPEEAGAPAPAHGPEDRVRLRDRWLGTRPAQLLATGGQLCTEPDRVAELAAVPLPFHVLSGTYDDTWPVPLLDEMALRLDARRTVVAGAEHSPNTDQPLPTARALADFWDDHPVPPRPYVR
Guillaume Brault et al. (2012) β€” Isolation and Characterization of EstC, a New Cold-Active Esterase from Streptomyces coelicolor A3(2)
PLoS ONE  Β· doi:10.1371/journal.pone.0032041 β†—  Β· Stability - Incubation
16 measurements ▼
Database ID
UniProt: Q9FBJ3 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 10% 100 108 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 30% 100 2 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 10% 100 96 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 30% 100 59 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 105 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30% 100 104 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethylformamide (DMF) 10% 100 110 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethylformamide (DMF) 30% 100 56 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 10% 100 110 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 30% 100 1 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 10% 100 107 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 30% 100 75 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Propanol 10% 100 90 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Propanol 30% 100 16 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Tetrahydrofuran (THF) 10% 100 25 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Tetrahydrofuran (THF) 30% 100 4 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 0.1% Triton X-100 (v/v) Incubation: 8.5, Assay: 8.5 Incubation: 25Β°C, Assay: 25Β°C 0.05 mM p-Nitrophenyl valerate p-Nitrophenol , Pentanoic Acid β€” β€” Water-incubated control (in %), activity in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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