Elastase from Pseudomonas aeruginosa A2

Enzyme Description

Species
Pseudomonas aeruginosa A2 β†—
Extremophile
No
EC Number
3.4.24.26 β†—

Sequence

Length: 301 amino acids
AEAGGPGGNQKIGKYTYGSDYGPLIVNDRCEMDDGNVITVDMNGSTNDSKTTPFRFACPTNTYKQVNGAYSPLNDAHFFGGVVFNLYRDWFGTSPLTHKLYMKVHYGRSVENAYWDGTAMLFGDGATMFYPLVSLDVAAHEVSHGFTEQNSGLIYRGQSGGMNEAFSDMAGEAAEFYMRGKNDFLIGYDIKKGSGALRYMDQPSRDGRSIDNASQYYNGIDVHHSSGVYNRAFYLLANSPGWDTRKAFEVFVDANRYYWTATSNYNSGACGVISSAQNRNYSAADVTRAFSTVGVTCPSAL
Olfa Ghorbel-Bellaaj et al. (2012) β€” Pseudomonas aeruginosa A2 elastase: Purification, characterization and biotechnological applications
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2012.01.038 β†—  Β· Stability - Half-life
8 measurements ▼
Database ID
UniProt: Q3Y6H8 β†—
Sequence Annotation
Explicit - Provided (starts from residue 198 from UniProt for mature form)
Protein Source
Purified, bacterium Pseudomonas aeruginosa A2

Experimental Data (8 measurements)

8 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Half-life Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Acetone 50% 29.0 0.5 days Incubation: 33 (?) mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Chloroform 50% 29.0 1.4 days Incubation: 33 (?) mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Ethanol 50% 29.0 2.3 days Incubation: 33 (?) mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Dimethylformamide (DMF) 50% 29.0 11.0 days Incubation: 33 (?) mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Methanol 50% 29.0 14.0 days Incubation: 33 (?) mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase n-Hexane 50% 29.0 20.0 days Incubation: 33 (?) mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Diethyl Ether 50% 29.0 >90.0 days Incubation: 33 (?) mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 29.0 >90.0 days Incubation: 33 (?) mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)

Visualization : Stability β€” Half-life

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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