Alcohol dehydrogenase ADH12 from Haloarcula marismortui
Enzyme Description
Species
Extremophile
Yes
"extreme halophilic" organism
EC Number
Sequence
MFLYLYSIFGNCFHPITRPGGCDRRHENGFPQPTRNGSMRAAIYRGPGDITVEEVPRPEIESPTDAIVRVTHTAVCGSDLWFYRGQSDREEGSRVGHEPMGIVEEVGDDVRSVEPGDRVFAPFVVSCGRCEFCRKGLHTSCVNGDSWGGDNGGGQGEYVRATEADGTLVRVPDRHADDEDTLEAILPLTDVMGTGHHAAVSAGVSEGDTCIVVGDGAVGLCGVLAARRLGAERIIAMGHHEDRLELAESFGATETVAARGQDAVDAADDLTNGGANHVLECVGAASAMETAIDVCRPGGTVGYVGVPHGLDDSGLDMFSLFGDNIGLNGGIAPVRAYADELLADVLQGTLDPSPIFTKTVDLNGVPEGYQAMDEREAIKVLVKL
Leanne M. Timpson et al. (2011)
β Characterization of alcohol dehydrogenase (ADH12) from Haloarcula marismortui, an extreme halophile from the Dead Sea
Extremophiles
Β· doi:10.1007/s00792-011-0405-0 β
Β· Stability - Incubation
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Database ID
UniProt: Q5V676 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host archaeon Haloferax volcanii
Experimental Data (3 measurements)
3 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 10% | 100 | 0 | % | Incubation: ?, Assay: 50 mM glycine-NaOH, 2M KCl | Incubation: ?, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Non-incubated control (in %), control and residual activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent, as well as for control |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 78 | % | Incubation: ?, Assay: 50 mM glycine-NaOH, 2M KCl | Incubation: ?, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Non-incubated control (in %), control and residual activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent, as well as for control |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Acetonitrile | 10% | 100 | 69 | % | Incubation: ?, Assay: 50 mM glycine-NaOH, 2M KCl | Incubation: ?, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Non-incubated control (in %), control and residual activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent, as well as for control |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.