Endoglucanase EGtf1 from Thermobifida fusca
Enzyme Description
Sequence
MLRRPRSRSPLVALTAATCRVALGGTAVPAQADEVNQIRNGDFSSGTAPWWGTENIQLNVTDGMLCVDVPGGTVNPWDVIIGQDDIPLIEGESYAFSFTASSTVPVSIRALVQEPVEPWTTQMDERALLGPEAETYEFVFTSNVDWDDAQVAFQIGGSDEPWTFCLDDVALLGRAEPPVYEPDTGPRVRVNQVGYLPHGPKKATVVTDATSALTWELADADGNVVASGQTKPHGADSSSGLNVHTVDFSSYTTKGSDYTLTVDGETSYPFDIDESVYEELRVDALSFYYPQRSGIEILDSIAPGYGRPAGHIGVPPNQGDTDVPCAPGTCDYSLDVSGGWYDAGDHGKYVVNGGISVHQIMSIYERSQLADTAQPDKLADSTLRLPETGNGVPDVLDEARWEMEFLLKMQVPEGEPLAGMAHHKIHDEQWTGLPLLPSADPQPRYLQPPSTAATLNLAATAAQCARVFEPFDEDFAAECLAAAETAWDAAKANPNIYAPAFGEGGGPYNDNNVTDEFYWAAAELFLTTGKEEYRDAVTSSPLHTDDEEVFRDGAFDWGWTAALARLQLATIPNDLADRDRVRQSVVDAADMYLANVETSPWGLAYKPNNGVFVWGSNSAVLNNMVILAVAFDLTGDTKYRDGVLEGMDYIFGRNALNQSYVTGYGDKDSRNQHSRWYAHQLDPRLPNPPKGTLAGGPNSDSTTWDPVAQSKLTGCAPQMCYIDHIESWSTNELTINWNAPLSWIASFIADQDDAGEPGGEEPGPGDDETPPSKPGNLKASDITATSATLTWDASTDNVGVVGYKVSLVRDGDAEEVGTTAQTSYTLTGLSADQEYTVQVVAYDAAGNLSTPATVTFTTEKEDETPTPSASCAVTYQTNDWPGGFTASVTLTNTGSTPWDSWELRFTFPSGQTVSHGWSANWQQSGSDVTATSLPWNGSVPPGGGSVNIGFNGTWGGSNTKPEKFTVNGAVCSIG
Li-Huan Qiu et al. (2011)
β Thermostable Bacterial Endoglucanases Mined from Swiss-Prot Database
Applied Biochemistry and Biotechnology
Β· doi:10.1007/s12010-011-9368-y β
Β· Activity + Stability - Incubation
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Database ID
UniProt: Q08166 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (11 measurements)
11 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Incubation | Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Methanol | 50% | 100 | 96 | % | Incubation: 50 mM citrate buffer, Assay: 50 mM citrate | Incubation: 5, Assay: 5 | Incubation: 50Β°C, Assay: 50Β°C | 0.25% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | Incubation: 900 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about control type |
| Activity + Stability - Incubation | Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Ethanol | 50% | 100 | 79 | % | Incubation: 50 mM citrate buffer, Assay: 50 mM citrate | Incubation: 5, Assay: 5 | Incubation: 50Β°C, Assay: 50Β°C | 0.25% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | Incubation: 900 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about control type |
| Activity + Stability - Incubation | Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Isopropanol | 50% | 100 | 61 | % | Incubation: 50 mM citrate buffer, Assay: 50 mM citrate | Incubation: 5, Assay: 5 | Incubation: 50Β°C, Assay: 50Β°C | 0.25% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | Incubation: 900 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about control type |
| Activity + Stability - Incubation | Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Acetone | 50% | 100 | 55 | % | Incubation: 50 mM citrate buffer, Assay: 50 mM citrate | Incubation: 5, Assay: 5 | Incubation: 50Β°C, Assay: 50Β°C | 0.25% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | Incubation: 900 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about control type |
| Activity + Stability - Incubation | Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50% | 100 | 55 | % | Incubation: 50 mM citrate buffer, Assay: 50 mM citrate | Incubation: 5, Assay: 5 | Incubation: 50Β°C, Assay: 50Β°C | 0.25% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | Incubation: 900 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about control type |
| Activity + Stability - Incubation | Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 50% | 100 | 29 | % | Incubation: 50 mM citrate buffer, Assay: 50 mM citrate | Incubation: 5, Assay: 5 | Incubation: 50Β°C, Assay: 50Β°C | 0.25% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | Incubation: 900 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about control type |
| Activity + Stability - Incubation | Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 50% | 100 | 25 | % | Incubation: 50 mM citrate buffer, Assay: 50 mM citrate | Incubation: 5, Assay: 5 | Incubation: 50Β°C, Assay: 50Β°C | 0.25% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | Incubation: 900 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about control type |
| Activity + Stability - Incubation | Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | 1-Propanol | 50% | 100 | 55 | % | Incubation: 50 mM citrate buffer, Assay: 50 mM citrate | Incubation: 5, Assay: 5 | Incubation: 50Β°C, Assay: 50Β°C | 0.25% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | Incubation: 900 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about control type |
| Activity + Stability - Incubation | Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | 1-Hexanol | 50% | 100 | 107 | % | Incubation: 50 mM citrate buffer, Assay: 50 mM citrate | Incubation: 5, Assay: 5 | Incubation: 50Β°C, Assay: 50Β°C | 0.25% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | Incubation: 900 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about control type |
| Activity + Stability - Incubation | Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | 1-Octanol | 50% | 100 | 143 | % | Incubation: 50 mM citrate buffer, Assay: 50 mM citrate | Incubation: 5, Assay: 5 | Incubation: 50Β°C, Assay: 50Β°C | 0.25% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | Incubation: 900 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about control type |
| Activity + Stability - Incubation | Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | 1-Dodecanol | 50% | 100 | 212 | % | Incubation: 50 mM citrate buffer, Assay: 50 mM citrate | Incubation: 5, Assay: 5 | Incubation: 50Β°C, Assay: 50Β°C | 0.25% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | Incubation: 900 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about control type |
Visualization : Activity + Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.