Lipase LipBL from Marinobacter lipolyticus SM19

Enzyme Description

Species
Marinobacter lipolyticus SM19 β†—
Extremophile
Yes "halophilic" organism
EC Number
3.1.1.3 β†—

Sequence

Length: 404 amino acids
MRESKQVTGLSSGHLTHIEDHLDRRYIQPGKLPGALTLVARRGEIAYLKAQGLMDVERNKPVCRDTVFRIYSMTKPITSIAMMQLYEQGRFLLDDPVHKYIPAWKNLRVYNSGVYPNFLTTPATSTMTIRDLFTHMSGLTYGFMNRTNVDAAYRELKLDGGRNLTLEALVGHLAEVPLEFSPGTAWNYSVSTDVLGYLVQLLADQPFDEYLREHIFEPLAMPDTGFHVRDDQLNRFAACYQYDPVDQFKLQDDPQTSPFRDKRRFLSGGGGLVSTIDDYFHFAQALCQGGEFGGRRIIGRKTLEFMRRNHLPGNQDLPGLSVGPFSETPYAGTGFGLGFSVKTDVAKSQINGSVGEYGWGGLASTNFIIDPVEELVVIFMTQLIPSSTYPIRQELRAIVNGALV
Dolores PΓ©rez et al. (2011) β€” A Novel Halophilic Lipase, LipBL, Showing High Efficiency in the Production of Eicosapentaenoic Acid (EPA)
PLoS ONE  Β· doi:10.1371/journal.pone.0023325 β†—  Β· Activity + Stability - Incubation
11 measurements ▼
Database ID
UniProt: E5BB89 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli XL1-Blue

Experimental Data (11 measurements)

11 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30% 100.0 97.5 % Incubation: 25 mM sodium phosphate buffer, Assay: 25 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "in the presence of a rotary shaker" Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Dimethylformamide (DMF) 30% 100.0 98.3 % Incubation: 25 mM sodium phosphate buffer, Assay: 25 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "in the presence of a rotary shaker" Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Methanol 30% 100.0 120.5 % Incubation: 25 mM sodium phosphate buffer, Assay: 25 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "in the presence of a rotary shaker" Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Acetonitrile 30% 100.0 27.7 % Incubation: 25 mM sodium phosphate buffer, Assay: 25 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "in the presence of a rotary shaker" Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Ethanol 30% 100.0 107.8 % Incubation: 25 mM sodium phosphate buffer, Assay: 25 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "in the presence of a rotary shaker" Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Acetone 30% 100.0 91.3 % Incubation: 25 mM sodium phosphate buffer, Assay: 25 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "in the presence of a rotary shaker" Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Isopropanol 30% 100.0 98.4 % Incubation: 25 mM sodium phosphate buffer, Assay: 25 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "in the presence of a rotary shaker" Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent 1-Propanol 30% 100.0 35.1 % Incubation: 25 mM sodium phosphate buffer, Assay: 25 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "in the presence of a rotary shaker" Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Diethyl Ether 30% 100.0 115.1 % Incubation: 25 mM sodium phosphate buffer, Assay: 25 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "in the presence of a rotary shaker" Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Toluene 5% 100.0 104.8 % Incubation: 25 mM sodium phosphate buffer, Assay: 25 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "in the presence of a rotary shaker" Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent
Activity + Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent n-Hexane 5% 100.0 94.1 % Incubation: 25 mM sodium phosphate buffer, Assay: 25 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 25Β°C, Assay: 25Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "in the presence of a rotary shaker" Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent

Visualization : Activity + Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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