7Ξ±-hydroxysteroid dehydrogenase from Bacteroides fragilis ATCC 25285
Enzyme Description
Extremophile
No
but "thermostable" enzyme
EC Number
Sequence
MNRFENKIIIITGAAGGIGASTTRRIVSEGGKVVIADYSREKADQFAAELSNSGADVRPVYFSATELKSCKELITFTMKEYGQIDVLVNNVGGTNPRRDTNIETLDMDYFDEAFHLNLSCTMYLSQLVIPIMSTQGGGNIVNVASISGITADSNGTLYGASKAGVINLTKYIATQTGKKNIRCNAVAPGLILTPAALNNLNEEVRKIFLGQCATPYLGEPQDVAATIAFLASEDARYITGQTIVVDGGLTIHNPTINLV
Yang Liu et al. (2011)
β The catalytic promiscuity of a microbial 7Ξ±-hydroxysteroid dehydrogenase. Reduction of non-steroidal carbonyl compounds
Steroids
Β· doi:10.1016/j.steroids.2011.05.001 β
Β· Activity + Stability - Conversion
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Database ID
UniProt: Q5LA59 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli
Experimental Data (7 measurements)
7 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Conversion | Conversion after incubation (12 hours at 30Β°C-) in the presence of organic solvent, measured by HPLC | Dimethyl Sulfoxide (DMSO) | 10% | 93 | 90 | % | 100 mM potassium phosphate buffer, 18 mg glucose, 0.35 U glucose dehydrogenase | 7 | 30Β°C | 0.02mmol/1ml Benzaldehyde | Benzyl Alcohol | 2 mg NAD | Yes, "the mixture was shaken" | Classical aqueous control (in %) |
| Activity + Stability - Conversion | Conversion after incubation (12 hours at 30Β°C-) in the presence of organic solvent, measured by HPLC | Methanol | 10% | 93 | 94 | % | 100 mM potassium phosphate buffer, 18 mg glucose, 0.35 U glucose dehydrogenase | 7 | 30Β°C | 0.02mmol/1ml Benzaldehyde | Benzyl Alcohol | 2 mg NAD | Yes, "the mixture was shaken" | Classical aqueous control (in %) |
| Activity + Stability - Conversion | Conversion after incubation (12 hours at 30Β°C-) in the presence of organic solvent, measured by HPLC | Isopropanol | 10% | 93 | 92 | % | 100 mM potassium phosphate buffer, 18 mg glucose, 0.35 U glucose dehydrogenase | 7 | 30Β°C | 0.02mmol/1ml Benzaldehyde | Benzyl Alcohol | 2 mg NAD | Yes, "the mixture was shaken" | Classical aqueous control (in %) |
| Activity + Stability - Conversion | Conversion after incubation (12 hours at 30Β°C-) in the presence of organic solvent, measured by HPLC | tert-Butyl Methyl Ether (MTBE) | 10% | 93 | 94 | % | 100 mM potassium phosphate buffer, 18 mg glucose, 0.35 U glucose dehydrogenase | 7 | 30Β°C | 0.02mmol/1ml Benzaldehyde | Benzyl Alcohol | 2 mg NAD | Yes, "the mixture was shaken" | Classical aqueous control (in %) |
| Activity + Stability - Conversion | Conversion after incubation (12 hours at 30Β°C-) in the presence of organic solvent, measured by HPLC | Ethyl Acetate | 10% | 93 | 79 | % | 100 mM potassium phosphate buffer, 18 mg glucose, 0.35 U glucose dehydrogenase | 7 | 30Β°C | 0.02mmol/1ml Benzaldehyde | Benzyl Alcohol | 2 mg NAD | Yes, "the mixture was shaken" | Classical aqueous control (in %) |
| Activity + Stability - Conversion | Conversion after incubation (12 hours at 30Β°C-) in the presence of organic solvent, measured by HPLC | Butyl Acetate | 10% | 93 | 63 | % | 100 mM potassium phosphate buffer, 18 mg glucose, 0.35 U glucose dehydrogenase | 7 | 30Β°C | 0.02mmol/1ml Benzaldehyde | Benzyl Alcohol | 2 mg NAD | Yes, "the mixture was shaken" | Classical aqueous control (in %) |
| Activity + Stability - Conversion | Conversion after incubation (12 hours at 30Β°C-) in the presence of organic solvent, measured by HPLC | Toluene | 10% | 93 | 85 | % | 100 mM potassium phosphate buffer, 18 mg glucose, 0.35 U glucose dehydrogenase | 7 | 30Β°C | 0.02mmol/1ml Benzaldehyde | Benzyl Alcohol | 2 mg NAD | Yes, "the mixture was shaken" | Classical aqueous control (in %) |
Visualization : Activity + Stability β Conversion
One bar per measurement. Colour = solvent, shade = solvent volume.