Family VII esterase (gene estDL30) from an alluvial soil metagenomic library

Enzyme Description

Species
Na (alluvial soil metagenomic library)
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 507 amino acids
MNVVESVPVLVEAPCGKLEGSRRGGLSSFKGIPFATARRWHMPERAQPWSGVRSARAPGAVAPQNPTPLEGLIASGGKNEQSEGCLFLNVWTPACDAAKRPVMVWIHGGAFSTGAGSIGLYSGKNLATVGDVVVVTINYRLSSLGFLRLTDITDGRIPSTGAEGIADQIAALSWVRDNIVAFGGDPGNVTIFGESAGAMSVACLLASPKARGLFHKAISQSGTAHIARPREHANRVAEVFLGHLGAAANDLERAPVEALLKAQADLTAEVDNKQDPHKLGTMALQPVVDGDVLPLWPIEAVRAGSAAGVPIIAGTTTEEWKLWTALDSKFHTMDEDKLARWAERMFGDEAAALLAADREGSPYERYVQMQTDRAFREPTRRMLAAQSVHAPVYEYAFDWRSPAMGGAFGACHAMELGFVFGTHSLPGADNFFGKGPEAEAISLAMIQAWTSFARTGVPKADGVDAWPQWSKASPAAMVFGADSRAAHVSCFEPRQAWAALPDRFVGP
Weixin Tao et al. (2011) β€” Isolation and characterization of a family VII esterase derived from alluvial soil metagenomic library
The Journal of Microbiology  Β· doi:10.1007/s12275-011-1102-5 β†—  Β· Activity - Classical
12 measurements ▼
Database ID
UniParc: UPI00021A8D14 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Methanol 15% 100.0 107.0 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Methanol 30% 100.0 63.0 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Ethanol 15% 100.0 57.0 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Ethanol 30% 100.0 18.0 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Isopropanol 15% 100.0 25.0 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Isopropanol 30% 100.0 3.0 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetone 15% 100.0 18.0 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetone 30% 100.0 4.0 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 15% 100.0 22.0 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 30% 100.0 0.4 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15% 100.0 58.0 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30% 100.0 40.0 % 50 mM Tris-HCl buffer, 100 mM NaCl, 0.3% Triton X-100 8 25Β°C 2 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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