Manganese-dependent serine protease from Bacillus pumilus TMS55

Enzyme Description

Species
Bacillus pumilus TMS55 β†—
Extremophile
No
EC Number
3.4.- β†—

Sequence

Length: 383 amino acids
MCVKEKNVMTSVLLAVPLLFSAGFGGSMANAETVSKTDSEKSYIVGFKASATTNSSKKQAVIQNGGKLEKQYRLINAAQVKMSEQAAKKLEHDPSIAYVEEDHKAEAYAQTVPYGIPQIKAPAVHAQGYKGANVKVAVLDTGIHAAHPDLNAAGGASFVPSEPNATQDFQSHGTHVAGTIAALDNTIGVLGVAPSASLYAVKALDRNGDGQYSWIISGIEWAVANNMDVINMSLGGASGSTALKNAVDTANSRGVVAVAAAGNSGSSGSRSTVGYPAKYESTIAVANVNSNNVRNSSSSAGPELDVSAPGTSILSTVPSSGYTSYTGTSMASPHVAGAAALILSKNPNLTNSQVRQRLENTATPLGDSFYYGKGLINVQAASN
Kalibulla Syed Ibrahim et al. (2011) β€” Purification and Characterization of Manganese-Dependent Alkaline Serine
Journal of Microbiology and Biotechnology  Β· doi:10.4014/jmb.1009.09001 β†—  Β· Stability - Incubation
9 measurements ▼
Database ID
UniProt: B9VNT4 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Bacillus pumilus TMS55

Experimental Data (9 measurements)

9 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at room temperature) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 440 nm) in aqueous phase Ethanol 25% 100 13 % Incubation: 50 mM phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 9.5, Assay: 7.8 Incubation: Room temperature, Assay: 37Β°C 1.2% (w/v) Azocasein Azo-peptides , Peptides β€” Yes, "constant shaking" Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at room temperature) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 440 nm) in aqueous phase Acetone 25% 100 21 % Incubation: 50 mM phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 9.5, Assay: 7.8 Incubation: Room temperature, Assay: 37Β°C 1.2% (w/v) Azocasein Azo-peptides , Peptides β€” Yes, "constant shaking" Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at room temperature) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 440 nm) in aqueous phase Pyridine 25% 100 0 % Incubation: 50 mM phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 9.5, Assay: 7.8 Incubation: Room temperature, Assay: 37Β°C 1.2% (w/v) Azocasein Azo-peptides , Peptides β€” Yes, "constant shaking" Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at room temperature) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 440 nm) in aqueous phase 1-Butanol 25% 100 18 % Incubation: 50 mM phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 9.5, Assay: 7.8 Incubation: Room temperature, Assay: 37Β°C 1.2% (w/v) Azocasein Azo-peptides , Peptides β€” Yes, "constant shaking" Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at room temperature) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 440 nm) in aqueous phase Benzene 25% 100 95 % Incubation: 50 mM phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 9.5, Assay: 7.8 Incubation: Room temperature, Assay: 37Β°C 1.2% (w/v) Azocasein Azo-peptides , Peptides β€” Yes, "constant shaking" Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at room temperature) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 440 nm) in aqueous phase Chloroform 25% 100 90 % Incubation: 50 mM phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 9.5, Assay: 7.8 Incubation: Room temperature, Assay: 37Β°C 1.2% (w/v) Azocasein Azo-peptides , Peptides β€” Yes, "constant shaking" Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at room temperature) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 440 nm) in aqueous phase Toluene 25% 100 100 % Incubation: 50 mM phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 9.5, Assay: 7.8 Incubation: Room temperature, Assay: 37Β°C 1.2% (w/v) Azocasein Azo-peptides , Peptides β€” Yes, "constant shaking" Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at room temperature) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 440 nm) in aqueous phase Xylene 25% 100 84 % Incubation: 50 mM phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 9.5, Assay: 7.8 Incubation: Room temperature, Assay: 37Β°C 1.2% (w/v) Azocasein Azo-peptides , Peptides β€” Yes, "constant shaking" Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at room temperature) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 440 nm) in aqueous phase n-Hexane 25% 100 100 % Incubation: 50 mM phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 9.5, Assay: 7.8 Incubation: Room temperature, Assay: 37Β°C 1.2% (w/v) Azocasein Azo-peptides , Peptides β€” Yes, "constant shaking" Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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