Serine protease from Engyodontium album BTMFS10
Enzyme Description
Sequence
MRLSILLGLLPLALGAPAVDAVEQRSEPAPLIEARGEMIADKYIVKLKEGSAFASLDATMEKLSGKADHVYKNIFKGFAASLDEKMVEVLRAHPDVEYIEQDAIVNINAEQRNAPWGLARISSTSPGTSSYRYDESAGQGTCVCVIDTGVEASHPEFEGRAQMVKTYYSSSRDGNGHGTHCAGTIGSRTYGVAKKTQIFGVKVLNDQGSSQYSTIISGMDFVANDYRNRNCPNGVVASMSIGGGYSSSVNSAAANLQQSGVMVAVAAGNNNADARNYSPASESSICTVGATDRYDRRSSFSNYGSVLDIFAPGTDIISTWIGGTTRIISGTSMATPHVAGLAAYFMTLGRATASNACRYIAQTANQGDLSGIPFGTVNLLAYNNYQG
Sreeja Chellappan et al. (2010)
β Characterization of an extracellular alkaline serine protease from marine Engyodontium album BTMFS10
Journal of Industrial Microbiology & Biotechnology
Β· doi:10.1007/s10295-010-0914-3 β
Β· Activity + Stability - Incubation
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Database ID
UniProt: B2FDP6 β
Sequence Annotation
Explicit - Provided
Protein Source
Purified, fungus Engyodontium album BTMFS10
Experimental Data (5 measurements)
5 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 28Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in the presence of organic solvent | Isopropanol | 10% | 100 | 7 | % | Incubation: 0.1 M phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 30Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Non-incubated control (in %), residual activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 28Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in the presence of organic solvent | Acetonitrile | 10% | 100 | 32 | % | Incubation: 0.1 M phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 30Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Non-incubated control (in %), residual activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 28Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in the presence of organic solvent | Petroleum Ether | 10% | 100 | >65 | % | Incubation: 0.1 M phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 30Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Non-incubated control (in %), residual activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 28Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100 | >65 | % | Incubation: 0.1 M phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 30Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Non-incubated control (in %), residual activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 28Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in the presence of organic solvent | Diethyl Ether | 10% | 100 | >90 | % | Incubation: 0.1 M phosphate buffer, Assay: 50 mM phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 30Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Non-incubated control (in %), residual activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
Visualization : Activity + Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.