Organic-solvent tolerant protease from Geomicrobium sp. EMB2

Enzyme Description

Species

Geomicrobium sp. EMB2 ↗

Extremophile

Yes "moderately halophilic" organism

EC Number

3.4.- ↗

Sequence

Length: 375 amino acids

MHPTRYRELNHKSSKKVAGVSANSRAPSIGLYGALVLIPAFETDKSSLGLGIDRPQQAKRPTTPTQIPNDLDCQNAENRHINPSTTIQRSQTLSATAVPIQSSASSNDVHRKFSTCFGSTMQTVASFYQKAQGPAWYIRKHRRLGQLNISHHTPCHSRRFGNVSLALTDVHSCSSIHDFRVGHDELTDVQLCLEIDPAKMSQPLPSLAILRSYSRRSFAKTSDRTIANPYSALGTYRSAFFMHKALLSATERSILRRYPVSAYNGQSCFSSLECIFGYSLQTVRRRKKSRWQLAALTYSHITMIEPLIPGILQSIMNEKNLQRMQQDDLLEGLCDPYVSFDVSQPFSDFRHVQYAESCETHSITRHHIKQIYSMN

Ram Karan et al. (2011) — A novel organic solvent tolerant protease from a newly isolated Geomicrobium sp. EMB2 (MTCC 10310): production optimization by response surface methodology

New Biotechnology · doi:10.1016/j.nbt.2010.10.007 ↗ · Stability - Incubation

18 measurements ▼

Database ID

UniProt: D7RJV5 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, bacterium Geomicrobium sp. EMB2

Experimental Data (18 measurements)

18 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Ethanol	75%	100	39	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Dodecane	75%	100	101	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Decane	75%	100	100	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Isooctane	75%	100	97	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Heptane	75%	100	96	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Cyclohexane	75%	100	98	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Toluene	75%	100	101	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Benzene	75%	100	91	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	1-Butanol	75%	100	68	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Ethanol	25%	100	50	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Dodecane	25%	100	108	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Decane	25%	100	105	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Isooctane	25%	100	103	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Heptane	25%	100	102	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Cyclohexane	25%	100	105	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Toluene	25%	100	109	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Benzene	25%	100	103	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	1-Butanol	25%	100	83	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids , Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent

Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Ethanol	75%	100	39	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Dodecane	75%	100	101	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Decane	75%	100	100	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Isooctane	75%	100	97	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Heptane	75%	100	96	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Cyclohexane	75%	100	98	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Toluene	75%	100	101	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Benzene	75%	100	91	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	1-Butanol	75%	100	68	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Ethanol	25%	100	50	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Dodecane	25%	100	108	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Decane	25%	100	105	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Isooctane	25%	100	103	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Heptane	25%	100	102	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Cyclohexane	25%	100	105	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Toluene	25%	100	109	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Benzene	25%	100	103	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent
Stability - Incubation	Activity after incubation (72 hours at ?°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	1-Butanol	25%	100	83	%	Incubation: ?, Assay: 20 mM borax-NaOH, 5% NaCl w/v	Incubation: ?, Assay: 10	Incubation: ?, Assay: 37°C	0.5% Casein	free amino acids, Peptides	—	—	Water-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether residual activity assay was with or without organic solvent

Visualization : Stability — Incubation

Ram Karan et al. (2011)

One bar per measurement. Colour = solvent, shade = solvent volume.

R. Karan et al. (2011) — Stability of haloalkaliphilic Geomicrobium sp. protease modulated by salt

Biochemistry (Moscow) · doi:10.1134/S0006297911060095 ↗ · Stability - Half-life

132 measurements ▼

Database ID

UniProt: D7RJV5 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, bacterium Geomicrobium sp. EMB2

Experimental Data (132 measurements)

132 measurements — page 1 of 7

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	1.0	0.3	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Ethanol	25%	1.0	0.2	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	1-Butanol	25%	1.0	0.3	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Dichloromethane	25%	1.0	0.4	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Benzene	25%	1.0	0.4	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Toluene	25%	1.0	0.6	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Hexane	25%	1.0	0.5	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Heptane	25%	1.0	0.5	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Octane	25%	1.0	0.6	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Decane	25%	1.0	0.7	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Dodecane	25%	1.0	0.7	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	1.0	0.2	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Ethanol	50%	1.0	0.1	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	1-Butanol	50%	1.0	0.2	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Dichloromethane	50%	1.0	0.3	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Benzene	50%	1.0	0.3	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	Toluene	50%	1.0	0.4	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Hexane	50%	1.0	0.4	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Heptane	50%	1.0	0.4	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase	n-Octane	50%	1.0	0.3	days	Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl	Incubation: 8, Assay: 10	Incubation: 30°C, Assay: 50°C	0.5% (w/v) Casein	free amino acids , Peptides	—	Incubation: 200 rpm	Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25%

1.0

0.3

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

25%

1.0

0.2

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

25%

1.0

0.3

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

25%

1.0

0.4

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

25%

1.0

0.4

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

25%

1.0

0.6

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

25%

1.0

0.5

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

25%

1.0

0.5

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

25%

1.0

0.6

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

25%

1.0

0.7

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

25%

1.0

0.7

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50%

1.0

0.2

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

50%

1.0

0.1

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

50%

1.0

0.2

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

50%

1.0

0.3

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

50%

1.0

0.3

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

50%

1.0

0.4

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

50%

1.0

0.4

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

50%

1.0

0.4

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

50%

1.0

0.3

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

50%

1.0

0.5

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

50%

1.0

0.6

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

75%

1.0

0.2

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

75%

1.0

0.0

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

75%

1.0

0.1

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

75%

1.0

0.2

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

75%

1.0

0.3

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

75%

1.0

0.2

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

75%

1.0

0.3

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

75%

1.0

0.2

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

75%

1.0

0.2

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

75%

1.0

0.4

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

75%

1.0

0.5

days

Incubation: 50 mM Tris-HCl, with 0% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25%

5.9

3.1

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

25%

5.9

1.0

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

25%

5.9

1.9

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

25%

5.9

8.5

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

25%

5.9

13.1

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

25%

5.9

18.7

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

25%

5.9

15.3

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

25%

5.9

14.7

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

25%

5.9

15.6

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

25%

5.9

19.6

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

25%

5.9

20.4

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50%

5.9

2.4

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

50%

5.9

0.7

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

50%

5.9

1.5

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

50%

5.9

5.3

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

50%

5.9

8.5

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

50%

5.9

14.3

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

50%

5.9

9.2

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

50%

5.9

8.3

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

50%

5.9

9.2

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

50%

5.9

14.1

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

50%

5.9

15.1

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

75%

5.9

1.5

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

75%

5.9

0.4

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

75%

5.9

1.0

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

75%

5.9

3.4

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

75%

5.9

4.2

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

75%

5.9

9.1

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

75%

5.9

5.1

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

75%

5.9

4.2

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

75%

5.9

6.3

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

75%

5.9

10.6

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

75%

5.9

12.2

days

Incubation: 50 mM Tris-HCl, with 2% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25%

24.6

5.3

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

25%

24.6

1.8

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

25%

24.6

3.3

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

25%

24.6

13.1

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

25%

24.6

32.5

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

25%

24.6

44.3

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

25%

24.6

42.5

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

25%

24.6

38.4

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

25%

24.6

39.1

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

25%

24.6

40.2

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

25%

24.6

41.6

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50%

24.6

4.0

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

50%

24.6

1.4

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

50%

24.6

2.2

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

50%

24.6

8.2

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

50%

24.6

25.8

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

50%

24.6

39.8

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

50%

24.6

36.1

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

50%

24.6

32.6

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

50%

24.6

34.7

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

50%

24.6

35.7

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

50%

24.6

37.2

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

75%

24.6

3.1

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

75%

24.6

0.8

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

75%

24.6

1.5

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

75%

24.6

6.0

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

75%

24.6

21.5

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

75%

24.6

36.4

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

75%

24.6

33.7

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

75%

24.6

28.1

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

75%

24.6

30.3

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

75%

24.6

32.1

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

75%

24.6

34.3

days

Incubation: 50 mM Tris-HCl, with 5% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25%

22.5

6.2

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

25%

22.5

2.1

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

25%

22.5

3.6

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

25%

22.5

14.3

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

25%

22.5

32.8

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

25%

22.5

44.4

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

25%

22.5

41.3

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

25%

22.5

38.1

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

25%

22.5

38.5

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

25%

22.5

39.3

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

25%

22.5

40.7

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50%

22.5

5.4

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

50%

22.5

1.6

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

50%

22.5

3.1

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

50%

22.5

11.7

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

50%

22.5

29.1

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

50%

22.5

42.1

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

50%

22.5

39.0

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

50%

22.5

36.1

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

50%

22.5

35.9

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

50%

22.5

37.2

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

50%

22.5

38.4

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

75%

22.5

4.3

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Ethanol

75%

22.5

1.3

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

1-Butanol

75%

22.5

2.4

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Dichloromethane

75%

22.5

8.1

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Benzene

75%

22.5

26.3

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

Toluene

75%

22.5

39.6

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Hexane

75%

22.5

37.8

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Heptane

75%

22.5

34.2

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Octane

75%

22.5

33.6

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Decane

75%

22.5

35.8

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase

n-Dodecane

75%

22.5

37.1

days

Incubation: 50 mM Tris-HCl, with 10% NaCl, Assay: 17 mM borax-NaOH buffer, 7 mM Tris-HCl

Incubation: 8, Assay: 10

Incubation: 30°C, Assay: 50°C

0.5% (w/v) Casein

free amino acids, Peptides

—

Incubation: 200 rpm

Classical aqueous control (in days)

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Visualization : Stability — Half-life

R. Karan et al. (2011)

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Organic-solvent tolerant protease from Geomicrobium sp. EMB2

Enzyme Description

Sequence

Experimental Data (18 measurements)

Visualization : Stability — Incubation

Experimental Data (132 measurements)

Visualization : Stability — Half-life

Structure

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