DNA polymerase eta from Homo sapiens
Enzyme Description
Sequence
MATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGLPQGPTTAEETVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLTKDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTVIKNCNTSGIQTEWSPPLTMLFLCATKFSASAPSSSTDITSFLSSDPSSLPKVPVTSSEAKTQGSGPAVTATKKATTSLESFFQKAAERQKVKEASLSSLTAPTQAPMSNSPSKPSLPFQTSQSTGTEPFFKQKSLLLKQKQLNNSSVSSPQQNPWSNCKALPNSLPTEYPGCVPVCEGVSKLEESSKATPAEMDLAHNSQSMHASSASKSVLEVTQKATPNPSLLAAEDQVPCEKCGSLVPVWDMPEHMDYHFALELQKSFLQPHSSNPQVVSAVSHQGKRNPKSPLACTNKRPRPEGMQTLESFFKPLTH
Sayo Kashiwagi et al. (2010)
β Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana
Journal of Nucleic Acids
Β· doi:10.4061/2010/701472 β
Β· Activity - Classical
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Database ID
UniProt: Q9Y253 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli Rosetta2 (DE3)
Experimental Data (9 measurements)
9 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 81 | % | 40 mM Tris-HCl buffer, 1mM MgClβ, 10 mM DTT, 60 mM KCl, 2.5% glycerol | 8 | 37Β°C | 40 nM 32P-labeled primer-templates, 100 mM dNTPs | DNA double strand incorporating dCTPs | β | β | Classical aqueous control (in %) |
| Activity - Classical | Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry | Ethanol | 20% | 100 | 79 | % | 40 mM Tris-HCl buffer, 1mM MgClβ, 10 mM DTT, 60 mM KCl, 2.5% glycerol | 8 | 37Β°C | 40 nM 32P-labeled primer-templates, 100 mM dNTPs | DNA double strand incorporating dCTPs | β | β | Classical aqueous control (in %) |
| Activity - Classical | Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry | Isopropanol | 20% | 100 | 26 | % | 40 mM Tris-HCl buffer, 1mM MgClβ, 10 mM DTT, 60 mM KCl, 2.5% glycerol | 8 | 37Β°C | 40 nM 32P-labeled primer-templates, 100 mM dNTPs | DNA double strand incorporating dCTPs | β | β | Classical aqueous control (in %) |
| Activity - Classical | Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry | Dimethyl Sulfoxide (DMSO) | 40% | 100 | 16 | % | 40 mM Tris-HCl buffer, 1mM MgClβ, 10 mM DTT, 60 mM KCl, 2.5% glycerol | 8 | 37Β°C | 40 nM 32P-labeled primer-templates, 100 mM dNTPs | DNA double strand incorporating dCTPs | β | β | Classical aqueous control (in %) |
| Activity - Classical | Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry | Dimethyl Sulfoxide (DMSO) | 5% | 100 | 89 | % | 40 mM Tris-HCl buffer, 1mM MgClβ, 10 mM DTT, 60 mM KCl, 2.5% glycerol | 8 | 37Β°C | 40 nM 32P-labeled primer-templates, 100 mM dNTPs | DNA double strand incorporating dCTPs | β | β | Classical aqueous control (in %) |
| Activity - Classical | Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 62 | % | 40 mM Tris-HCl buffer, 1mM MgClβ, 10 mM DTT, 60 mM KCl, 2.5% glycerol | 8 | 37Β°C | 40 nM 32P-labeled primer-templates, 100 mM dNTPs | DNA double strand incorporating dCTPs | β | β | Classical aqueous control (in %) |
| Activity - Classical | Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 49 | % | 40 mM Tris-HCl buffer, 1mM MgClβ, 10 mM DTT, 60 mM KCl, 2.5% glycerol | 8 | 37Β°C | 40 nM 32P-labeled primer-templates, 100 mM dNTPs | DNA double strand incorporating dCTPs | β | β | Classical aqueous control (in %) |
| Activity - Classical | Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry | Dimethyl Sulfoxide (DMSO) | 30% | 100 | 21 | % | 40 mM Tris-HCl buffer, 1mM MgClβ, 10 mM DTT, 60 mM KCl, 2.5% glycerol | 8 | 37Β°C | 40 nM 32P-labeled primer-templates, 100 mM dNTPs | DNA double strand incorporating dCTPs | β | β | Classical aqueous control (in %) |
| Activity - Classical | Relative activity (primer elongation of a G template by incorporation of dCMP) measured by radiochemistry | Dimethyl Sulfoxide (DMSO) | 40% | 100 | 5 | % | 40 mM Tris-HCl buffer, 1mM MgClβ, 10 mM DTT, 60 mM KCl, 2.5% glycerol | 8 | 37Β°C | 40 nM 32P-labeled primer-templates, 100 mM dNTPs | DNA double strand incorporating dCTPs | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.