Protease from Geomicrobium sp. EMB2

Enzyme Description

Species
Geomicrobium sp. EMB2 β†—
Extremophile
No but "moderately haloalkaliphilic bacterium" and "organic-solvent tolerant" enzyme
EC Number
3.4.- β†—

Sequence

Length: 375 amino acids
MHPTRYRELNHKSSKKVAGVSANSRAPSIGLYGALVLIPAFETDKSSLGLGIDRPQQAKRPTTPTQIPNDLDCQNAENRHINPSTTIQRSQTLSATAVPIQSSASSNDVHRKFSTCFGSTMQTVASFYQKAQGPAWYIRKHRRLGQLNISHHTPCHSRRFGNVSLALTDVHSCSSIHDFRVGHDELTDVQLCLEIDPAKMSQPLPSLAILRSYSRRSFAKTSDRTIANPYSALGTYRSAFFMHKALLSATERSILRRYPVSAYNGQSCFSSLECIFGYSLQTVRRRKKSRWQLAALTYSHITMIEPLIPGILQSIMNEKNLQRMQQDDLLEGLCDPYVSFDVSQPFSDFRHVQYAESCETHSITRHHIKQIYSMN
Ram Karan et al. (2010) β€” Purification and characterization of a solvent-stable protease from Geomicrobium sp. EMB2
Environmental Technology  Β· doi:10.1080/09593331003674556 β†—  Β· Stability - Incubation
9 measurements ▼
Database ID
UniProt: D7RJV5 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Geomicrobium sp. EMB2

Experimental Data (9 measurements)

9 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hours at 30Β°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase 1-Butanol 50% 97 34 % Incubation: 50 mM Tris-HCl, 5% NaCl w/v, Assay: 20 mM borax-NaOH, 7 mM Tris-HCl, 2% NaCl w/v Incubation: 8, Assay: 10 Incubation: 30Β°C, Assay: 50Β°C 0.5% Casein free amino acids , Peptides β€” Incubation: 200 rpm Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Dichloromethane 50% 97 98 % Incubation: 50 mM Tris-HCl, 5% NaCl w/v, Assay: 20 mM borax-NaOH, 7 mM Tris-HCl, 2% NaCl w/v Incubation: 8, Assay: 10 Incubation: 30Β°C, Assay: 50Β°C 0.5% Casein free amino acids , Peptides β€” Incubation: 200 rpm Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Benzene 50% 97 118 % Incubation: 50 mM Tris-HCl, 5% NaCl w/v, Assay: 20 mM borax-NaOH, 7 mM Tris-HCl, 2% NaCl w/v Incubation: 8, Assay: 10 Incubation: 30Β°C, Assay: 50Β°C 0.5% Casein free amino acids , Peptides β€” Incubation: 200 rpm Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Toluene 50% 97 123 % Incubation: 50 mM Tris-HCl, 5% NaCl w/v, Assay: 20 mM borax-NaOH, 7 mM Tris-HCl, 2% NaCl w/v Incubation: 8, Assay: 10 Incubation: 30Β°C, Assay: 50Β°C 0.5% Casein free amino acids , Peptides β€” Incubation: 200 rpm Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Cyclohexane 50% 97 133 % Incubation: 50 mM Tris-HCl, 5% NaCl w/v, Assay: 20 mM borax-NaOH, 7 mM Tris-HCl, 2% NaCl w/v Incubation: 8, Assay: 10 Incubation: 30Β°C, Assay: 50Β°C 0.5% Casein free amino acids , Peptides β€” Incubation: 200 rpm Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase n-Heptane 50% 97 124 % Incubation: 50 mM Tris-HCl, 5% NaCl w/v, Assay: 20 mM borax-NaOH, 7 mM Tris-HCl, 2% NaCl w/v Incubation: 8, Assay: 10 Incubation: 30Β°C, Assay: 50Β°C 0.5% Casein free amino acids , Peptides β€” Incubation: 200 rpm Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Isooctane 50% 97 128 % Incubation: 50 mM Tris-HCl, 5% NaCl w/v, Assay: 20 mM borax-NaOH, 7 mM Tris-HCl, 2% NaCl w/v Incubation: 8, Assay: 10 Incubation: 30Β°C, Assay: 50Β°C 0.5% Casein free amino acids , Peptides β€” Incubation: 200 rpm Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase n-Decane 50% 97 125 % Incubation: 50 mM Tris-HCl, 5% NaCl w/v, Assay: 20 mM borax-NaOH, 7 mM Tris-HCl, 2% NaCl w/v Incubation: 8, Assay: 10 Incubation: 30Β°C, Assay: 50Β°C 0.5% Casein free amino acids , Peptides β€” Incubation: 200 rpm Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C) measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase n-Dodecane 50% 97 128 % Incubation: 50 mM Tris-HCl, 5% NaCl w/v, Assay: 20 mM borax-NaOH, 7 mM Tris-HCl, 2% NaCl w/v Incubation: 8, Assay: 10 Incubation: 30Β°C, Assay: 50Β°C 0.5% Casein free amino acids , Peptides β€” Incubation: 200 rpm Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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