Laccase from Fusarium solani MAS2

Enzyme Description

Species
Fusarium solani MAS2 β†—
Extremophile
No
EC Number
1.10.3.2 β†—

Sequence

Length: 588 amino acids
MVNSGTQHGGTFNLKGLGKLSQIKTNGKSLLGTLLAPLLPLFLANNPTPNGYPWSTLTSHTNYYDDHPNTGVIRRYDFSVSRGTIAPDGYELPVILVNGQFPGPTIEANWGDTIQVTVNNDIEDEGLALHWHGLQQKKTPWEDGVPGVTQCPIPPGESFTYQFVADMYGTTWYHSHYSAQYSAGLFGPLVIYGPREKKNYDIDVGPVILSDWYHKEYFDLVEETMRPNAPGPVFSDSNLINGKMNFNCSSVQEGDKTPCNNNAGISKFRFKRGKTHRLRLINAGGEGLQRFSIDEHTMTVIANDFVQVQPYDTKVVTLGIGQRTDVLVKADGDLDAYWMRSNISSKCSLSHAPDAVAAIFYDGADHDKPPESQPWDIPDPGTCANDDLELTKPVMQLPLPKADLTLDLDVELHVNASNVTLWRFGGVDFRANYNSPTLLLSKLGNHSFEEQWNVRNLGDAKSVRVNVINKTPVAHPIHLHGFNMYVLHEGPGTWDGTIINEHNPQRRDVVQLQANGHLVIQFDAAKNPGVWPFHCHIAWHVSAGFLVQFLTNPDKVEKLRIPNVVAETCRQWGTWTSSNIPAQIDSGL
Yi-Rui Wu et al. (2010) β€” Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2
Bioresource Technology  Β· doi:10.1016/j.biortech.2010.07.091 β†—  Β· Activity - Classical
15 measurements ▼
Database ID
UniProt: V9XWW2 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, fungus Fusarium solani MAS2

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetone 5% 100 58 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetone 20% 100 9 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetone 50% 100 2 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 5% 100 75 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 20% 100 25 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 50% 100 0 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 100 66 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100 12 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 50% 100 0 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 5% 100 70 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 20% 100 18 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 50% 100 0 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Methanol 5% 100 80 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Methanol 20% 100 38 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Methanol 50% 100 1 % 100 mM sodium tartrate buffer 4.5 30Β°C 0.1 mM ABTS ABTS radical cation reaction products β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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