Xyloglucan xyloglucosyl transferases from Hordeum vulgare (barley)

Enzyme Description

Species

Extremophile

EC Number

2.4.1.207 ↗

Sequence

Length: 285 amino acids

MASGPSRTVPCSVLPLLLLLAGVARAAGNFYQDVDITWGDGRGKILGGGDLLTLSLDRASGSGFQSKNQYLYGRFDMQIKLVPGDSAGTVATFYLSSQGSAHDEIDFEFLGNASGQPYTVHTNVYSQGKGGREQQFRMWFDPTADFHTYSVLWNPTHILFYVDGTPIREHRNREAATGVPYLRSQAMRVYASVWDAEEWATQGGRVRTDWSRAPFVASYKGLAASGCASQDAAACANSNGAWMYQELDATALDRLQWVQKNYMIYNYCTDTWRFKDGAPPECASK

Gustav Vaaje-Kolstad et al. (2010) — Xyloglucan xyloglucosyl transferases from barley (Hordeum vulgare L.) bind oligomeric and polymeric xyloglucan molecules in their acceptor binding sites

Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2010.04.001 ↗ · Activity - Michaelis-Menten

30 measurements ▼

Database ID

UniProt: B1P1S7 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host yeast Pichia pastoris

Experimental Data (30 measurements)

30 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Michaelis-Menten	Km (mg/ml) for TXG measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	0.2	0.3	mg/ml	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in mg/ml)
Activity - Michaelis-Menten	kcat/Km (ml/(µgram*s)) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Acetone	20%	0.2	0.02	ml/(µgram * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in ml/(microgram * s))
Activity - Michaelis-Menten	kcat/Km (ml/(µgram*s)) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	1,4-Butanediol	20%	0.2	0.2	ml/(µgram * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in ml/(microgram * s))
Activity - Michaelis-Menten	kcat/Km (ml/(µgram*s)) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Methanol	20%	0.2	0.6	ml/(µgram * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in ml/(microgram * s))
Activity - Michaelis-Menten	kcat/Km (ml/(µgram*s)) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Glycerol	20%	0.2	0.2	ml/(µgram * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in ml/(microgram * s))
Activity - Michaelis-Menten	kcat/Km (ml/(µgram*s)) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	0.2	0.1	ml/(µgram * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in ml/(microgram * s))
Activity - Michaelis-Menten	kcat (/s) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Acetone	20%	0.046	0.002	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	1,4-Butanediol	20%	0.046	0.036	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Methanol	20%	0.046	0.055	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Glycerol	20%	0.046	0.05	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	0.046	0.043	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	Km (mg/ml) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Acetone	20%	0.2	0.1	mg/ml	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in mg/ml)
Activity - Michaelis-Menten	Km (mg/ml) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	1,4-Butanediol	20%	0.2	0.2	mg/ml	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in mg/ml)
Activity - Michaelis-Menten	Km (mg/ml) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Methanol	20%	0.2	0.1	mg/ml	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in mg/ml)
Activity - Michaelis-Menten	Km (mg/ml) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Glycerol	20%	0.2	0.3	mg/ml	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in mg/ml)
Activity - Michaelis-Menten	Km (µM) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	10.6	7.7	µM	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in microM)
Activity - Michaelis-Menten	kcat/Km (1/(µM*s)) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Acetone	20%	0.005	0.0002	1/(µM * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/(microM * s))
Activity - Michaelis-Menten	kcat/Km (1/(µM*s)) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	1,4-Butanediol	20%	0.005	0.005	1/(µM * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/(microM * s))
Activity - Michaelis-Menten	kcat/Km (1/(µM*s)) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Methanol	20%	0.005	0.004	1/(µM * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/(microM * s))
Activity - Michaelis-Menten	kcat/Km (1/(µM*s)) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Glycerol	20%	0.005	0.004	1/(µM * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG , TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/(microM * s))

Activity - Michaelis-Menten	Km (mg/ml) for TXG measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	0.2	0.3	mg/ml	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in mg/ml)
Activity - Michaelis-Menten	kcat/Km (ml/(µgram*s)) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Acetone	20%	0.2	0.02	ml/(µgram * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in ml/(µgram * s))
Activity - Michaelis-Menten	kcat/Km (ml/(µgram*s)) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	1,4-Butanediol	20%	0.2	0.2	ml/(µgram * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in ml/(µgram * s))
Activity - Michaelis-Menten	kcat/Km (ml/(µgram*s)) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Methanol	20%	0.2	0.6	ml/(µgram * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in ml/(µgram * s))
Activity - Michaelis-Menten	kcat/Km (ml/(µgram*s)) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Glycerol	20%	0.2	0.2	ml/(µgram * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in ml/(µgram * s))
Activity - Michaelis-Menten	kcat/Km (ml/(µgram*s)) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	0.2	0.1	ml/(µgram * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in ml/(µgram * s))
Activity - Michaelis-Menten	kcat (/s) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Acetone	20%	0.046	0.002	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	1,4-Butanediol	20%	0.046	0.036	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Methanol	20%	0.046	0.055	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Glycerol	20%	0.046	0.05	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	0.046	0.043	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	Km (mg/ml) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Acetone	20%	0.2	0.1	mg/ml	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in mg/ml)
Activity - Michaelis-Menten	Km (mg/ml) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	1,4-Butanediol	20%	0.2	0.2	mg/ml	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in mg/ml)
Activity - Michaelis-Menten	Km (mg/ml) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Methanol	20%	0.2	0.1	mg/ml	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in mg/ml)
Activity - Michaelis-Menten	Km (mg/ml) for TXG substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Glycerol	20%	0.2	0.3	mg/ml	50 mM ammonium acetate buffer	6	30°C	fixed concentration xyloglucan-derived heptaitol [1-3H]-XXXGol, variable concentration tamarind xyloglucan	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in mg/ml)
Activity - Michaelis-Menten	Km (µM) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	10.6	7.7	µM	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in µM)
Activity - Michaelis-Menten	kcat/Km (1/(µM*s)) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Acetone	20%	0.005	0.0002	1/(µM * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/(µM * s))
Activity - Michaelis-Menten	kcat/Km (1/(µM*s)) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	1,4-Butanediol	20%	0.005	0.005	1/(µM * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/(µM * s))
Activity - Michaelis-Menten	kcat/Km (1/(µM*s)) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Methanol	20%	0.005	0.004	1/(µM * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/(µM * s))
Activity - Michaelis-Menten	kcat/Km (1/(µM*s)) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Glycerol	20%	0.005	0.004	1/(µM * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/(µM * s))
Activity - Michaelis-Menten	kcat/Km (1/(µM*s)) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	0.005	0.002	1/(µM * s)	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/(µM * s))
Activity - Michaelis-Menten	kcat (/s) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Acetone	20%	0.058	0.004	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	1,4-Butanediol	20%	0.058	0.03	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Methanol	20%	0.058	0.026	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Glycerol	20%	0.058	0.027	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	0.058	0.013	1/s	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	Km (µM) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Acetone	20%	10.6	17.2	µM	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in µM)
Activity - Michaelis-Menten	Km (µM) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	1,4-Butanediol	20%	10.6	6.2	µM	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in µM)
Activity - Michaelis-Menten	Km (µM) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Methanol	20%	10.6	7.4	µM	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in µM)
Activity - Michaelis-Menten	Km (µM) for XXXGol substrate measured by radiometry (scintillation counting of [1-3H]-labelled xyloglucan-derived heptaitol XXXGol incorporation into TXG) in the presence of organic solvent	Glycerol	20%	10.6	6.9	µM	50 mM ammonium acetate buffer	6	30°C	fixed concentration tamarind xyloglucan, variable concentration xyloglucan-derived heptaitol [1-3H]-XXXGol	shortened TXG, TXG–Glc–β(1→4)–XXXGol	—	—	Classical aqueous control (in µM)

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Visualization : Activity — Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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