Transaminase ArS from Arthrobacter citreus

Enzyme Description

Species
Arthrobacter citreus β†—
Extremophile
No
EC Number
2.6.1.- β†—

Sequence

Length: 485 amino acids
MGLTVQKINWEQVKEWDRKYLMRTFSTQNEYQPVPIESTEGDYLITPGGTRLLDFFNQLCCVNLGQKNQKVNAAIKEALDRYGFVWDTYATDYKAKAAKIIIEDILGDEDWPGKVRFVSTGSEAVETALNIARLYTNRPLVVTREHDYHGWTGGAATVTRLRSFRSGLVGENSESFSAQIPGSSCSSAVLMAPSSNTFQDSNGNYLKDENGELLSVKYTRRMIENYGPEQVAAVITEVSQGVGSTMPPYEYVPQIRKMTKELGVLWISDEVLTGFGRTGKWFGYQHYGVQPDIITMGKGLSSSSLPAGAVVVSKEIAAFMDKHRWESVSTYAGHPVAMAAVCANLEVMMEENLVEQAKNSGEYIRSKLELLQEKHKSIGNFDGYGLLWIVDIVNAKTKTPYVKLDRNFRHGMNPNQIPTQIIMEKALEKGVLIGGAMPNTMRIGASLNVSRGDIDKAMDALDYALDYLESGEWQQSALEHHHHHH
Frieda A. Sorgenfrei et al. (2024) β€” Solvent concentration at 50% protein unfolding may reform enzyme stability ranking and process window identification
Nature Communications  Β· doi:10.1038/s41467-024-49774-0 β†—  Β· Stability - C50
15 measurements ▼
Database ID
UniProt: A0A1C7D191 β†—
Sequence Annotation
Explicit - Provided PDB Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF Dimethylformamide (DMF) β€” β€” 31 % (v/v) 50 mM sodium phosphate 7.4 60 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF Dimethylformamide (DMF) β€” β€” 15 % (v/v) 50 mM sodium phosphate 7.4 70 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF Dimethyl Sulfoxide (DMSO) β€” β€” 45 % (v/v) 50 mM sodium phosphate 7.4 60 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF Dimethyl Sulfoxide (DMSO) β€” β€” 31 % (v/v) 50 mM sodium phosphate 7.4 70 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF Ethanol β€” β€” 25 % (v/v) 50 mM sodium phosphate 7.4 50 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF Ethanol β€” β€” 17 % (v/v) 50 mM sodium phosphate 7.4 60 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF Ethanol β€” β€” 6 % (v/v) 50 mM sodium phosphate 7.4 70 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF Methanol β€” β€” 47 % (v/v) 50 mM sodium phosphate 7.4 45 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF Methanol β€” β€” 44 % (v/v) 50 mM sodium phosphate 7.4 50 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF Methanol β€” β€” 24 % (v/v) 50 mM sodium phosphate 7.4 60 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF Methanol β€” β€” 9 % (v/v) 50 mM sodium phosphate 7.4 70 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 23 % (v/v) 50 mM sodium phosphate 7.4 45 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 18 % (v/v) 50 mM sodium phosphate 7.4 50 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 10 % (v/v) 50 mM sodium phosphate 7.4 60 β€” β€” β€” β€” Not applicable control (in % (v/v))
Stability - C50 Volume of organic solvent that leads to half-unfolding, measured by nanoDSF 1-Propanol β€” β€” 4 % (v/v) 50 mM sodium phosphate 7.4 70 β€” β€” β€” β€” Not applicable control (in % (v/v))

Visualization : Stability β€” C50

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Structure

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