Lipase from Pseudomonas aeruginosa CS-2

Enzyme Description

Species
Pseudomonas aeruginosa CS-2 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 317 amino acids
MKKKSLLPLGLAIGLASLAASPLIQASTYTQTKYPIVLAHGMLGFDNILGVDYWFGIPSALRRDGAQVYVTEVSQLDTSEVRGEQLLQQVEEIVALSGQPKVNLIGHSHGGPTIRYVAAVRPDLIASATSVGAPHKGSDTADFLRQIPPGSAGEAILSGLVNSLGALISFLSSGSTGTQNSLGSLESLNSEGAARFNAKYPQGVPTSACGEGAYKVNGVSYYSWSGSSPLTNFLDPSDAFLGASSLTFKNGTANDGLVGTCSSHLGMVIRDNYRMNHLDEVNQVFGLTSLFETSPVSVYRQHANRLKNASLWDPGRG
Ren Peng et al. (2009) β€” Purification and Characterization of an Organic Solvent-Tolerant Lipase from Pseudomonas aeruginosa CS-2
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-009-8841-3 β†—  Β· Stability - Incubation
20 measurements ▼
Database ID
UniProt: D8L9W5 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Pseudomonas aeruginosa CS-2

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (9 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100.0 56.0 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (9 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isooctane 50% 100.0 57.4 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (9 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Petroleum Ether 50% 100.0 72.7 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (9 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 50% 100.0 69.4 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (9 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 50% 100.0 52.2 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (9 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Benzene 50% 100.0 62.2 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (9 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 50% 100.0 130.6 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (9 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 50% 100.0 22.4 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (9 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 50% 100.0 8.9 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (9 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 50% 100.0 6.9 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100.0 65.7 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isooctane 50% 100.0 75.0 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Petroleum Ether 50% 100.0 79.4 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 50% 100.0 86.4 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 50% 100.0 67.1 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Benzene 50% 100.0 79.2 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 50% 100.0 139.8 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 50% 100.0 45.4 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 50% 100.0 13.5 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 50% 100.0 10.2 % Incubation: 20 mM Tris-HCl, Assay: 40 mM Tris-HCl, 20% Isopropanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 50Β°C 4 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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