Protease from Bacillus cereus SV1

Enzyme Description

Species
Bacillus cereus SV1 β†—
Extremophile
No but "oxidant- and solvent-stable" enzyme
EC Number
3.4.24.- β†—

Sequence

Length: 566 amino acids
MKKKSLALVLATGMAVTTFGGTGSAFADSKNVLSTKKYNETVQSPEFVSGDLTEATGKKAESVVFDYLNAAKGDYKLGEKSAQDSFKVKQAKKDAVTDSTVLRLQQVYEGVPVWGSTQVAHVSKDGSLKVLSGTVAPDLDKKEKLKNKNKIEGAKAIEIAQKDLGVTPKYEVEPKADLYVYQNGEETTYAYVVNLNFLEPSPGNYYYFIEADSGKVLNKYNKLDHVANEDKSPVKQEAPKQEAKPAVKPVTGTNAVGTGKGVLGDTKSLNTTLSASSYYLQGNTRGATIFTYDAKNRSTLPGTLWVDADNVFNAAYDAAAVDAHYYAGKTYDYYKATFNRNSINDAGAPLKSTVHYGSKYNNAFWNGSQMVYGDGDGVTFTSLSGGIDVIGHELTHAVTEYSSDLIYQNESGALNEAISDVFGTLVEYYDNRNPDWEIGEDIYTPGKAGDALRSMSDPTKYGDPDHYSKRYTGTSDNGGVHTNSGIINKAAYLLANGGTHYGVTVNGIGKDKVGAIYYRANTQYFTQSTTFSQARAGLVQAAADLYGASSAEVAAVKQSYSAVGVN
Laila Manni et al. (2009) β€” An Oxidant- and Solvent-Stable Protease Produced by Bacillus cereus SV1: Application in the Deproteinization of Shrimp Wastes and as a Laundry Detergent Additive
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-009-8703-z β†—  Β· Stability - Incubation Stability - Half-life
20 measurements ▼
Database ID
UniProt: Q45KG7 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Bacillus cereus SV1

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 34.0 >60.0 days Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Ethanol 50% 34.0 >60.0 days Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Dimethylformamide (DMF) 50% 34.0 48.0 days Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase 1-Butanol 50% 34.0 18.0 days Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Acetonitrile 50% 34.0 27.0 days Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase n-Hexane 50% 34.0 1.5 days Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Acetone 50% 34.0 0.8 days Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Diethyl Ether 50% 34.0 >60.0 days Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Ethyl Acetate 50% 34.0 51.0 days Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Methanol 50% 34.0 >60.0 days Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Incubation Activity after incubation (60 days at 30Β°C) measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Ethyl Acetate 50% 34.45 45.0 % Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (60 days at 30Β°C) measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Diethyl Ether 50% 34.45 100.0 % Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (60 days at 30Β°C) measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Acetone 50% 34.45 0.0 % Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (60 days at 30Β°C) measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase n-Hexane 50% 34.45 0.0 % Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (60 days at 30Β°C) measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Acetonitrile 50% 34.45 0.0 % Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (60 days at 30Β°C) measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase 1-Butanol 50% 34.45 25.0 % Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (60 days at 30Β°C) measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Dimethylformamide (DMF) 50% 34.45 32.7 % Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (60 days at 30Β°C) measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Ethanol 50% 34.45 69.6 % Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (60 days at 30Β°C) measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 34.45 100.0 % Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (60 days at 30Β°C) measured by absorbance spectrophotometry (tyrosine absorbance measurement, 280 nm) in aqueous phase Methanol 50% 34.45 75.8 % Incubation: Culture medium supernatant, Assay: Tris-HCl buffer, 1 mM CaCl2, (g/L), SWP 10.0, ammonium sulfate 2.0, K2HPO4 0.5, KH2PO4 0.5, and MgSO4, 7 H2O 0.1 supernatant,50% supernatant Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 60Β°C 0.5% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Half-life

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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