Lipase (gene LipCst) from Caldanaerobacter subterraneus subsp. Tengcongensis

Enzyme Description

Species
Caldanaerobacter subterraneus subsp. Tengcongensis β†—
Extremophile
Yes "extreme thermophilic" organism
EC Number
3.1.1.3 β†—

Sequence

Length: 258 amino acids
MQKAVEFTYNRKTLRGMLHLPEGVSEKVPMVVMFHGFTGNKVESHFIFVKMSRALEKVGIGSVRFDFYGSGESDGDFSEMTFSGELEDARQILDFVKRQPTTDVERIGLLGLSMGGAIAGIIARERKEDVKALVLWAPAFNMPELIMGEGARQYGAIMESLGYVDIGGLKLDRAFVEDIAKFNIFELSRGYEGKVLIVHGTNDEAVEYRISDRILQEVYGDNAFRVTIEGADHTFKNLEWERKAIEESVKFFERELKG
Marina Royter et al. (2009) β€” Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOL1 and Caldanaerobacter subterraneus subsp. tengcongensis
Extremophiles  Β· doi:10.1007/s00792-009-0265-z β†—  Β· Stability - Incubation
18 measurements ▼
Database ID
UniProt: A0A4R2JD24 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli TunerTM(DE3)pLacI

Experimental Data (18 measurements)

18 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 99% 100 50 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Benzene 99% 100 4 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Toluene 99% 100 13 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Decanol 99% 100 26 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Pentanol 99% 100 51 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isooctane 99% 100 54 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 99% 100 72 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Heptane 99% 100 71 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexadecane 99% 100 63 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase tert-Butanol 99% 100 118 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethylformamide (DMF) 99% 100 49 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 99% 100 104 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 99% 100 1 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100 96 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Pyridine 99% 100 97 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 99% 100 111 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 99% 100 100 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (90 minutes at 30Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 99% 100 127 % Incubation: ?, Assay: 0.025 M Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 70Β°C 0.07 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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