Protease from Bacillus subtilis Rand

Enzyme Description

Species
Bacillus subtilis Rand β†—
Extremophile
No but "halotolerant" organism
EC Number
3.4.21.62 β†—

Sequence

Length: 381 amino acids
MRSKKLWISLLFAFTLIFTMAFSNMSAQAAGKNSEEKKYIVGFKQTMGAMSTAKKKDVISEKGGKVQKQFKYVNAAAATLDDKAVKELKKDPSVAYVEEDHVAHEYAQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTVAALNNTIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVASGIVVVAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGSELDVMAPGVSIQSTLPGGTYGSYNGTSMATPHVAGAAALILSKHPTWSNAQVRDRLESTATNLGSSFYYGKGLINVQAAAQ
Randa A Abusham et al. (2009) β€” Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain Rand
Microbial Cell Factories  Β· doi:10.1186/1475-2859-8-20 β†—  Β· Activity - Classical Stability - Incubation
20 measurements ▼
Database ID
UniProt: Q58GF0 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Bacillus subtilis Rand

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in the presence of organic solvent 1-Butanol 25% 37.0 23.0 U/ml 0.1 M Tris-HCl, 2 mM CaCl2 7 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in U/ml)
Activity - Classical Activity measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in the presence of organic solvent Benzene 25% 37.0 61.9 U/ml 0.1 M Tris-HCl, 2 mM CaCl2 7 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in U/ml)
Activity - Classical Activity measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in the presence of organic solvent Toluene 25% 37.0 45.7 U/ml 0.1 M Tris-HCl, 2 mM CaCl2 7 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in U/ml)
Activity - Classical Activity measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in the presence of organic solvent p-Xylene 25% 37.0 54.4 U/ml 0.1 M Tris-HCl, 2 mM CaCl2 7 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in U/ml)
Activity - Classical Activity measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in the presence of organic solvent n-Hexane 25% 37.0 47.22 U/ml 0.1 M Tris-HCl, 2 mM CaCl2 7 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in U/ml)
Activity - Classical Activity measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in the presence of organic solvent n-Decane 25% 37.0 53.4 U/ml 0.1 M Tris-HCl, 2 mM CaCl2 7 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in U/ml)
Activity - Classical Activity measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in the presence of organic solvent n-Dodecane 25% 37.0 65.0 U/ml 0.1 M Tris-HCl, 2 mM CaCl2 7 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in U/ml)
Activity - Classical Activity measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in the presence of organic solvent n-Tetradecane 25% 37.0 44.7 U/ml 0.1 M Tris-HCl, 2 mM CaCl2 7 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in U/ml)
Activity - Classical Activity measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in the presence of organic solvent n-Hexadecane 25% 37.0 61.9 U/ml 0.1 M Tris-HCl, 2 mM CaCl2 7 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in U/ml)
Activity - Classical Activity measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in the presence of organic solvent Pyridine 25% 37.0 22.66 U/ml 0.1 M Tris-HCl, 2 mM CaCl2 7 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in U/ml)
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in aqueous phase n-Hexadecane 25% 37.0 56.0 U/ml Incubation: Supernatant from culture medium with 0.5 g/l CaCl2.H2O, 2g/l KHSPO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l NaCl and 1% peptone, Assay: 0.1 M Tris-HCl, 2 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 50Β°C, Assay: 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” Incubation: 150 rpm Water-incubated control (in U/ml), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in aqueous phase n-Tetradecane 25% 37.0 56.4 U/ml Incubation: Supernatant from culture medium with 0.5 g/l CaCl2.H2O, 2g/l KHSPO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l NaCl and 1% peptone, Assay: 0.1 M Tris-HCl, 2 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 50Β°C, Assay: 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” Incubation: 150 rpm Water-incubated control (in U/ml), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in aqueous phase n-Dodecane 25% 37.0 55.7 U/ml Incubation: Supernatant from culture medium with 0.5 g/l CaCl2.H2O, 2g/l KHSPO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l NaCl and 1% peptone, Assay: 0.1 M Tris-HCl, 2 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 50Β°C, Assay: 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” Incubation: 150 rpm Water-incubated control (in U/ml), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in aqueous phase n-Decane 25% 37.0 77.4 U/ml Incubation: Supernatant from culture medium with 0.5 g/l CaCl2.H2O, 2g/l KHSPO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l NaCl and 1% peptone, Assay: 0.1 M Tris-HCl, 2 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 50Β°C, Assay: 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” Incubation: 150 rpm Water-incubated control (in U/ml), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in aqueous phase n-Hexane 25% 37.0 53.9 U/ml Incubation: Supernatant from culture medium with 0.5 g/l CaCl2.H2O, 2g/l KHSPO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l NaCl and 1% peptone, Assay: 0.1 M Tris-HCl, 2 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 50Β°C, Assay: 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” Incubation: 150 rpm Water-incubated control (in U/ml), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in aqueous phase p-Xylene 25% 37.0 49.6 U/ml Incubation: Supernatant from culture medium with 0.5 g/l CaCl2.H2O, 2g/l KHSPO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l NaCl and 1% peptone, Assay: 0.1 M Tris-HCl, 2 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 50Β°C, Assay: 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” Incubation: 150 rpm Water-incubated control (in U/ml), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in aqueous phase Toluene 25% 37.0 47.9 U/ml Incubation: Supernatant from culture medium with 0.5 g/l CaCl2.H2O, 2g/l KHSPO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l NaCl and 1% peptone, Assay: 0.1 M Tris-HCl, 2 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 50Β°C, Assay: 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” Incubation: 150 rpm Water-incubated control (in U/ml), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in aqueous phase Benzene 25% 37.0 72.7 U/ml Incubation: Supernatant from culture medium with 0.5 g/l CaCl2.H2O, 2g/l KHSPO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l NaCl and 1% peptone, Assay: 0.1 M Tris-HCl, 2 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 50Β°C, Assay: 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” Incubation: 150 rpm Water-incubated control (in U/ml), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in aqueous phase 1-Butanol 25% 37.0 38.3 U/ml Incubation: Supernatant from culture medium with 0.5 g/l CaCl2.H2O, 2g/l KHSPO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l NaCl and 1% peptone, Assay: 0.1 M Tris-HCl, 2 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 50Β°C, Assay: 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” Incubation: 150 rpm Water-incubated control (in U/ml), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (change in Azo-peptides absorbance measurement, 450 nm) in aqueous phase Pyridine 25% 37.0 0.0 U/ml Incubation: Supernatant from culture medium with 0.5 g/l CaCl2.H2O, 2g/l KHSPO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l NaCl and 1% peptone, Assay: 0.1 M Tris-HCl, 2 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 50Β°C, Assay: 50Β°C 0.5% Azocasein Azo-peptides , Peptides β€” Incubation: 150 rpm Water-incubated control (in U/ml), activity measured in aqueous phase | Uncertainty about whether activity was really measured in aqueous phase

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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