Lipase from Bacillus licheniformis RSP-09

Enzyme Description

Species
Bacillus licheniformis RSP-09 β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.1.1.3 β†—

Sequence

Length: 181 amino acids
AEHNPVVMVHGMGGASYNFASIKRYLVSQGWDQNQLFAIDFIDKTGNNLNNGPRLSRFVKDVLAKTGAKKVDIVAHSMGGANTLYYIKNLDGGDKIENVVTLGGANGLVSLRALPGTDPNQKILYTSVYSSADLIVVNSLSRLIGARNVLIHGVGHIGLLTSSQVKGYVKEGLNSGGQNTN
Bhawna Madan et al. (2009) β€” Overexpression, Purification and Characterization of Organic Solvent Stable Lipase from Bacillus licheniformis RSP-09
Microbial Physiology  Β· doi:10.1159/000208523 β†—  Β· Activity + Stability - Incubation
7 measurements ▼
Database ID
UniProt: B2L2K1 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (7 measurements)

7 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Activity after incubation (10 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isooctane 25% 100 54 % Incubation: 50 mM sodium carbonate buffer, Assay: 0.1 MM sodium carbonate buffer Incubation: 10, Assay: 10 Incubation: 37Β°C, Assay: 37Β°C 0.8 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Yes, "mixture incubated in rotary shaker" Non-incubated control (in %), residual activity | Uncertainty about whether organic solvent was present in the assay mixture, here indicated as yes ("lipase was found to retain 54% of its activity in [organic solvent]")
Activity + Stability - Incubation Activity after incubation (10 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 25% 100 65 % Incubation: 50 mM sodium carbonate buffer, Assay: 0.1 MM sodium carbonate buffer Incubation: 10, Assay: 10 Incubation: 37Β°C, Assay: 37Β°C 0.8 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Yes, "mixture incubated in rotary shaker" Non-incubated control (in %), residual activity | Uncertainty about whether organic solvent was present in the assay mixture, here indicated as yes ("lipase was found to retain 54% of its activity in [organic solvent]")
Activity + Stability - Incubation Activity after incubation (10 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Toluene 25% 100 58 % Incubation: 50 mM sodium carbonate buffer, Assay: 0.1 MM sodium carbonate buffer Incubation: 10, Assay: 10 Incubation: 37Β°C, Assay: 37Β°C 0.8 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Yes, "mixture incubated in rotary shaker" Non-incubated control (in %), residual activity | Uncertainty about whether organic solvent was present in the assay mixture, here indicated as yes ("lipase was found to retain 54% of its activity in [organic solvent]")
Activity + Stability - Incubation Activity after incubation (10 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Benzene 25% 100 53 % Incubation: 50 mM sodium carbonate buffer, Assay: 0.1 MM sodium carbonate buffer Incubation: 10, Assay: 10 Incubation: 37Β°C, Assay: 37Β°C 0.8 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Yes, "mixture incubated in rotary shaker" Non-incubated control (in %), residual activity | Uncertainty about whether organic solvent was present in the assay mixture, here indicated as yes ("lipase was found to retain 54% of its activity in [organic solvent]")
Activity + Stability - Incubation Activity after incubation (10 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 25% 100 23 % Incubation: 50 mM sodium carbonate buffer, Assay: 0.1 MM sodium carbonate buffer Incubation: 10, Assay: 10 Incubation: 37Β°C, Assay: 37Β°C 0.8 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Yes, "mixture incubated in rotary shaker" Non-incubated control (in %), residual activity | Uncertainty about whether organic solvent was present in the assay mixture, here indicated as yes ("lipase was found to retain 54% of its activity in [organic solvent]")
Activity + Stability - Incubation Activity after incubation (10 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethylformamide (DMF) 25% 100 57 % Incubation: 50 mM sodium carbonate buffer, Assay: 0.1 MM sodium carbonate buffer Incubation: 10, Assay: 10 Incubation: 37Β°C, Assay: 37Β°C 0.8 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Yes, "mixture incubated in rotary shaker" Non-incubated control (in %), residual activity | Uncertainty about whether organic solvent was present in the assay mixture, here indicated as yes ("lipase was found to retain 54% of its activity in [organic solvent]")
Activity + Stability - Incubation Activity after incubation (10 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25% 100 96 % Incubation: 50 mM sodium carbonate buffer, Assay: 0.1 MM sodium carbonate buffer Incubation: 10, Assay: 10 Incubation: 37Β°C, Assay: 37Β°C 0.8 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Yes, "mixture incubated in rotary shaker" Non-incubated control (in %), residual activity | Uncertainty about whether organic solvent was present in the assay mixture, here indicated as yes ("lipase was found to retain 54% of its activity in [organic solvent]")

Visualization : Activity + Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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