Serine hydroxymethyltransferase from Escherichia coli

Enzyme Description

Species
Escherichia coli β†—
Extremophile
No
EC Number
2.1.2.1 β†—

Sequence

Length: 417 amino acids
MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQANFAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDSINDEAVIERIKGKVLDICARYPVYA
Stefan Makart et al. (2007) β€” Towards preparative asymmetric synthesis of Ξ²-hydroxy-Ξ±-amino acids: l-allo-Threonine formation from glycine and acetaldehyde using recombinant GlyA
Journal of Biotechnology  Β· doi:10.1016/j.jbiotec.2007.05.008 β†—  Β· Activity - Classical Stability - Incubation
9 measurements ▼
Database ID
UniProt: P0A825 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli JM101 (pESM3)

Experimental Data (9 measurements)

9 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity in the presence of organic solvent measured by HPLC (product formation at 35Β°C) Methanol 10% 0.17 0.24 U/mg 50 mM sodium phosphate buffer 6.6 35Β°C 100 mM Acetaldehyde, 100 mM Glycine L-allo-Threonine 40 Β΅M PLP Yes, "continuously stirred batch", "shaking bath" Classical aqueous control (in U/mg)
Activity - Classical Activity in the presence of organic solvent measured by HPLC (product formation at 35Β°C) Methanol 20% 0.17 0.3 U/mg 50 mM sodium phosphate buffer 6.6 35Β°C 100 mM Acetaldehyde, 100 mM Glycine L-allo-Threonine 40 Β΅M PLP Yes, "continuously stirred batch", "shaking bath" Classical aqueous control (in U/mg)
Activity - Classical Activity in the presence of organic solvent measured by HPLC (product formation at 35Β°C) Methanol 30% 0.17 0.29 U/mg 50 mM sodium phosphate buffer 6.6 35Β°C 100 mM Acetaldehyde, 100 mM Glycine L-allo-Threonine 40 Β΅M PLP Yes, "continuously stirred batch", "shaking bath" Classical aqueous control (in U/mg)
Activity - Classical Activity in the presence of organic solvent measured by HPLC (product formation at 35Β°C) Acetonitrile 10% 0.17 0.2 U/mg 50 mM sodium phosphate buffer 6.6 35Β°C 100 mM Acetaldehyde, 100 mM Glycine L-allo-Threonine 40 Β΅M PLP Yes, "continuously stirred batch", "shaking bath" Classical aqueous control (in U/mg)
Activity - Classical Activity in the presence of organic solvent measured by HPLC (product formation at 35Β°C) Acetonitrile 20% 0.17 0.2 U/mg 50 mM sodium phosphate buffer 6.6 35Β°C 100 mM Acetaldehyde, 100 mM Glycine L-allo-Threonine 40 Β΅M PLP Yes, "continuously stirred batch", "shaking bath" Classical aqueous control (in U/mg)
Activity - Classical Activity in the presence of organic solvent measured by HPLC (product formation at 35Β°C) Acetonitrile 30% 0.17 0.15 U/mg 50 mM sodium phosphate buffer 6.6 35Β°C 100 mM Acetaldehyde, 100 mM Glycine L-allo-Threonine 40 Β΅M PLP Yes, "continuously stirred batch", "shaking bath" Classical aqueous control (in U/mg)
Stability - Incubation Activity after incubation (7 days at 24Β°C) measured in the presence of organic solvent by HPLC Methanol 10% 80.0 70.0 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 6.6, Assay: 6.6 Incubation: 25Β°C, Assay: 35Β°C 100 mM Acetaldehyde, 100 mM Glycine L-allo-Threonine 40 Β΅M PLP Yes, "shaking bath" Non-incubated control (in %) measured in the presence of organic solvent, residual activity measured in the presence of organic solvent
Stability - Incubation Activity after incubation (7 days at 24Β°C) measured in the presence of organic solvent by HPLC Acetonitrile 10% 80.0 70.0 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 6.6, Assay: 6.6 Incubation: 25Β°C, Assay: 35Β°C 100 mM Acetaldehyde, 100 mM Glycine L-allo-Threonine 40 Β΅M PLP Yes, "shaking bath" Non-incubated control (in %) measured in the presence of organic solvent, residual activity measured in the presence of organic solvent
Stability - Incubation Activity after incubation (7 days at 24Β°C) measured in the presence of organic solvent by HPLC Methanol 20% 80.0 83.0 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 6.6, Assay: 6.6 Incubation: 25Β°C, Assay: 35Β°C 100 mM Acetaldehyde, 100 mM Glycine L-allo-Threonine 40 Β΅M PLP Yes, "shaking bath" Non-incubated control (in %) measured in the presence of organic solvent, residual activity measured in the presence of organic solvent

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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