Chloroperoxidase from Leptoxyphium fumago
Enzyme Description
Sequence
MFSKVLPFVGAVAALPHSVRQEPGSGIGYPYDNNTLPYVAPGPTDSRAPCPALNALANHGYIPHDGRAISRETLQNAFLNHMGIANSVIELALTNAFVVCEYVTGSDCGDSLVNLTLLAEPHAFEHDHSFSRKDYKQGVANSNDFIDNRNFDAETFQTSLDVVAGKTHFDYADMNEIRLQRESLSNELDFPGWFTESKPIQNVESGFIFALVSDFNLPDNDENPLVRIDWWKYWFTNESFPYHLGWHPPSPAREIEFVTSASSAVLAASVTSTPSSLPSGAIGPGAEAVPLSFASTMTPFLLATNAPYYAQDPTLGPNDKREAAPAATTSMAVFKNPYLEAIGTQDIKNQQAYVSSKAAAMASAMAANKARNL
Jian-Zhong Liu et al. (2007)
β Improvement of activity and stability of chloroperoxidase by chemical modification
BMC Biotechnology
Β· doi:10.1186/1472-6750-7-23 β
Β· Stability - Incubation
▼
Database ID
UniProt: P04963 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (8 measurements)
8 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry (decrease in thioanisole absorbance measurement, 250 nm) in aqueous phase | Dimethylformamide (DMF) | 10% | 100 | 97 | % | Incubation: 0.1M sodium phosphate, Assay: 50 mM sodium citrate buffer | Incubation: 7.4, Assay: 5 | Incubation: 30Β°C, Assay: 30Β°C | HβOβ (Hydrogen Peroxide), 126 Β΅M Thioanisole | H2O (water) , Thioanisole sulfoxide | β | β | Non-incubated control (in %), residual activity | Residual activity measured in highly diluted presence of organic solvent, approximated as water) / slight doubt about the relative/residual activity |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry (decrease in thioanisole absorbance measurement, 250 nm) in aqueous phase | Dimethylformamide (DMF) | 20% | 100 | 89 | % | Incubation: 0.1M sodium phosphate, Assay: 50 mM sodium citrate buffer | Incubation: 7.4, Assay: 5 | Incubation: 30Β°C, Assay: 30Β°C | HβOβ (Hydrogen Peroxide), 126 Β΅M Thioanisole | H2O (water) , Thioanisole sulfoxide | β | β | Non-incubated control (in %), residual activity | Residual activity measured in highly diluted presence of organic solvent, approximated as water) / slight doubt about the relative/residual activity |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry (decrease in thioanisole absorbance measurement, 250 nm) in aqueous phase | Dimethylformamide (DMF) | 30% | 100 | 87 | % | Incubation: 0.1M sodium phosphate, Assay: 50 mM sodium citrate buffer | Incubation: 7.4, Assay: 5 | Incubation: 30Β°C, Assay: 30Β°C | HβOβ (Hydrogen Peroxide), 126 Β΅M Thioanisole | H2O (water) , Thioanisole sulfoxide | β | β | Non-incubated control (in %), residual activity | Residual activity measured in highly diluted presence of organic solvent, approximated as water) / slight doubt about the relative/residual activity |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry (decrease in thioanisole absorbance measurement, 250 nm) in aqueous phase | Dimethylformamide (DMF) | 40% | 100 | 3 | % | Incubation: 0.1M sodium phosphate, Assay: 50 mM sodium citrate buffer | Incubation: 7.4, Assay: 5 | Incubation: 30Β°C, Assay: 30Β°C | HβOβ (Hydrogen Peroxide), 126 Β΅M Thioanisole | H2O (water) , Thioanisole sulfoxide | β | β | Non-incubated control (in %), residual activity | Residual activity measured in highly diluted presence of organic solvent, approximated as water) / slight doubt about the relative/residual activity |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry (colorimetric assay, 4-Aminoantipyrine with oxidized phenol reaction product absorbance measurement at 510 nm) in aqueous phase | Dimethylformamide (DMF) | 10% | 100 | 89 | % | Incubation: 0.1M sodium phosphate, Assay: 50 mM sodium citrate buffer, 2.4 mM 4-Aminoantipyrine | Incubation: 7.4, Assay: 5 | Incubation: 30Β°C, Assay: 30Β°C | HβOβ (Hydrogen Peroxide), 10 mM Phenol | Catechol , H2O (water) | β | β | Non-incubated control (in %), residual activity | Residual activity measured in highly diluted presence of organic solvent, approximated as water) / slight doubt about the relative/residual activity |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry (colorimetric assay, 4-Aminoantipyrine with oxidized phenol reaction product absorbance measurement at 510 nm) in aqueous phase | Dimethylformamide (DMF) | 20% | 100 | 76 | % | Incubation: 0.1M sodium phosphate, Assay: 50 mM sodium citrate buffer, 2.4 mM 4-Aminoantipyrine | Incubation: 7.4, Assay: 5 | Incubation: 30Β°C, Assay: 30Β°C | HβOβ (Hydrogen Peroxide), 10 mM Phenol | Catechol , H2O (water) | β | β | Non-incubated control (in %), residual activity | Residual activity measured in highly diluted presence of organic solvent, approximated as water) / slight doubt about the relative/residual activity |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry (colorimetric assay, 4-Aminoantipyrine with oxidized phenol reaction product absorbance measurement at 510 nm) in aqueous phase | Dimethylformamide (DMF) | 30% | 100 | 62 | % | Incubation: 0.1M sodium phosphate, Assay: 50 mM sodium citrate buffer, 2.4 mM 4-Aminoantipyrine | Incubation: 7.4, Assay: 5 | Incubation: 30Β°C, Assay: 30Β°C | HβOβ (Hydrogen Peroxide), 10 mM Phenol | Catechol , H2O (water) | β | β | Non-incubated control (in %), residual activity | Residual activity measured in highly diluted presence of organic solvent, approximated as water) / slight doubt about the relative/residual activity |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry (colorimetric assay, 4-Aminoantipyrine with oxidized phenol reaction product absorbance measurement at 510 nm) in aqueous phase | Dimethylformamide (DMF) | 40% | 100 | 2 | % | Incubation: 0.1M sodium phosphate, Assay: 50 mM sodium citrate buffer, 2.4 mM 4-Aminoantipyrine | Incubation: 7.4, Assay: 5 | Incubation: 30Β°C, Assay: 30Β°C | HβOβ (Hydrogen Peroxide), 10 mM Phenol | Catechol , H2O (water) | β | β | Non-incubated control (in %), residual activity | Residual activity measured in highly diluted presence of organic solvent, approximated as water) / slight doubt about the relative/residual activity |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.