Protease from Bacillus pumilus 115b

Enzyme Description

Species
Bacillus pumilus β†—
Extremophile
No but organic-solvent tolerant enzyme
EC Number
3.4.- β†—

Sequence

Length: 383 amino acids
MCVKKKNVMTSVLLAVPLLFSAGFGGTMANAETVSKTDSEKSYIVGFKASATTNSSKKQAVIQNGGKLEKQYRLINAAQVKMSEQAAKKLEHDPSIAYVEEDHKAEAYAQTVPYGIPQIKAPAVHAQGYKGANVKVAVLDTGIHAAHPDLNVAGGASFVPSEPNATQDFQSHGTHVAGTIAALDNTIGVLGVAPNASLYAVKVLDRNGDGQYSWIISGIEWAVANNMDVINMSLGGPSGSTALKNAVDTANNRGVVVVAAAGNSGSSGSRSTVGYPAKYDSTIAVANVNSSNVRNSSSSAGPELDVSAPGTSILSTVPSSGYTSYTGTSMASPHVAGAAALILSKNPNLTNSQVRQRLENTATPLGDSFYYGKGLINVQAASN
Raja Noor Zaliha Raja Abd Rahman et al. (2007) β€” A new organic solvent tolerant protease from Bacillus pumilus 115b
Journal of Industrial Microbiology and Biotechnology  Β· doi:10.1007/s10295-007-0222-8 β†—  Β· Stability - Incubation
10 measurements ▼
Database ID
UniProt: Q5XPN0 β†—
Sequence Annotation
Explicit - provided (first 108 residues from UniProt sequence absent from the mature protein)
Protein Source
Purified, bacterium Bacillus pumilus 115b

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric assay, reaction of Folin-Ciocalteau reagant with tyrosines, 660 nm) in aqueous phase Ethyl Acetate 25% 100 10 % Incubation: Filtered supernatant from the culture medium, Assay: Filtered supernatant from the culture medium Incubation: 7, Assay: 7 Incubation: 37Β°C, Assay: 37Β°C 1% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Non-incubated control (in %), residual activity | Not sure if residual activity measured in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric assay, reaction of Folin-Ciocalteau reagant with tyrosines, 660 nm) in aqueous phase Benzene 25% 100 80 % Incubation: Filtered supernatant from the culture medium, Assay: Filtered supernatant from the culture medium Incubation: 7, Assay: 7 Incubation: 37Β°C, Assay: 37Β°C 1% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Non-incubated control (in %), residual activity | Not sure if residual activity measured in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric assay, reaction of Folin-Ciocalteau reagant with tyrosines, 660 nm) in aqueous phase 1-Heptanol 25% 100 54 % Incubation: Filtered supernatant from the culture medium, Assay: Filtered supernatant from the culture medium Incubation: 7, Assay: 7 Incubation: 37Β°C, Assay: 37Β°C 1% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Non-incubated control (in %), residual activity | Not sure if residual activity measured in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric assay, reaction of Folin-Ciocalteau reagant with tyrosines, 660 nm) in aqueous phase Toluene 25% 100 63 % Incubation: Filtered supernatant from the culture medium, Assay: Filtered supernatant from the culture medium Incubation: 7, Assay: 7 Incubation: 37Β°C, Assay: 37Β°C 1% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Non-incubated control (in %), residual activity | Not sure if residual activity measured in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric assay, reaction of Folin-Ciocalteau reagant with tyrosines, 660 nm) in aqueous phase Ethylbenzene 25% 100 90 % Incubation: Filtered supernatant from the culture medium, Assay: Filtered supernatant from the culture medium Incubation: 7, Assay: 7 Incubation: 37Β°C, Assay: 37Β°C 1% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Non-incubated control (in %), residual activity | Not sure if residual activity measured in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric assay, reaction of Folin-Ciocalteau reagant with tyrosines, 660 nm) in aqueous phase n-Hexane 25% 100 134 % Incubation: Filtered supernatant from the culture medium, Assay: Filtered supernatant from the culture medium Incubation: 7, Assay: 7 Incubation: 37Β°C, Assay: 37Β°C 1% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Non-incubated control (in %), residual activity | Not sure if residual activity measured in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric assay, reaction of Folin-Ciocalteau reagant with tyrosines, 660 nm) in aqueous phase 1-Decanol 25% 100 128 % Incubation: Filtered supernatant from the culture medium, Assay: Filtered supernatant from the culture medium Incubation: 7, Assay: 7 Incubation: 37Β°C, Assay: 37Β°C 1% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Non-incubated control (in %), residual activity | Not sure if residual activity measured in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric assay, reaction of Folin-Ciocalteau reagant with tyrosines, 660 nm) in aqueous phase Isooctane 25% 100 139 % Incubation: Filtered supernatant from the culture medium, Assay: Filtered supernatant from the culture medium Incubation: 7, Assay: 7 Incubation: 37Β°C, Assay: 37Β°C 1% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Non-incubated control (in %), residual activity | Not sure if residual activity measured in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric assay, reaction of Folin-Ciocalteau reagant with tyrosines, 660 nm) in aqueous phase n-Dodecane 25% 100 166 % Incubation: Filtered supernatant from the culture medium, Assay: Filtered supernatant from the culture medium Incubation: 7, Assay: 7 Incubation: 37Β°C, Assay: 37Β°C 1% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Non-incubated control (in %), residual activity | Not sure if residual activity measured in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric assay, reaction of Folin-Ciocalteau reagant with tyrosines, 660 nm) in aqueous phase n-Tetradecane 25% 100 248 % Incubation: Filtered supernatant from the culture medium, Assay: Filtered supernatant from the culture medium Incubation: 7, Assay: 7 Incubation: 37Β°C, Assay: 37Β°C 1% (w/v) Casein free amino acids , Peptides β€” Incubation: 150 rpm Non-incubated control (in %), residual activity | Not sure if residual activity measured in the presence of organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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