Cytochrome P450 2D6 from Homo sapiens

Enzyme Description

Species

Extremophile

EC Number

1.14.14.1 ↗

Sequence

Length: 497 amino acids

MGLEALVPLAVIVAIFLLLVDLMHRRQRWAARYPPGPLPLPGLGNLLHVDFQNTPYCFDQLRRRFGDVFSLQLAWTPVVVLNGLAAVREALVTHGEDTADRPPVPITQILGFGPRSQGVFLARYGPAWREQRRFSVSTLRNLGLGKKSLEQWVTEEAACLCAAFANHSGRPFRPNGLLDKAVSNVIASLTCGRRFEYDDPRFLRLLDLAQEGLKEESGFLREVLNAVPVLLHIPALAGKVLRFQKAFLTQLDELLTEHRMTWDPAQPPRDLTEAFLAEMEKAKGNPESSFNDENLRIVVADLFSAGMVTTSTTLAWGLLLMILHPDVQRRVQQEIDDVIGQVRRPEMGDQAHMPYTTAVIHEVQRFGDIVPLGVTHMTSRDIEVQGFRIPKGTTLITNLSSVLKDEAVWEKPFRFHPEHFLDAQGHFVKPEAFLPFSAGRRACLGEPLARMELFLFFTSLLQHFSFSVPTGQPRPSHHGVFAFLVSPSPYELCAVPR

Jin Zhao et al. (2007) — Activity of human P450 2D6 in biphasic solvent systems

Biotechnology and Bioengineering · doi:10.1002/bit.21449 ↗ · Activity + Stability - Product Formation

14 measurements ▼

Database ID

UniProt: P10635 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host cells not mentioned

Experimental Data (14 measurements)

14 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	Chloroform	33%	13.0	0.2	µM product formed after 1 hour	0.1 M potassium phosphate buffer	7.4	37°C	100 µM Dextromorphan	Dextrophan	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 1 hour)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	Dichloromethane	33%	13.0	0.0	µM product formed after 1 hour	0.1 M potassium phosphate buffer	7.4	37°C	100 µM Dextromorphan	Dextrophan	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 1 hour)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	1,1-Dichloroethane	33%	13.0	0.2	µM product formed after 1 hour	0.1 M potassium phosphate buffer	7.4	37°C	100 µM Dextromorphan	Dextrophan	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 1 hour)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	Butyl Ether	33%	13.0	2.3	µM product formed after 1 hour	0.1 M potassium phosphate buffer	7.4	37°C	100 µM Dextromorphan	Dextrophan	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 1 hour)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	Cyclohexane	33%	13.0	2.3	µM product formed after 1 hour	0.1 M potassium phosphate buffer	7.4	37°C	100 µM Dextromorphan	Dextrophan	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 1 hour)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	n-Pentane	33%	13.0	4.3	µM product formed after 1 hour	0.1 M potassium phosphate buffer	7.4	37°C	100 µM Dextromorphan	Dextrophan	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 1 hour)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	n-Hexane	33%	13.0	4.3	µM product formed after 1 hour	0.1 M potassium phosphate buffer	7.4	37°C	100 µM Dextromorphan	Dextrophan	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 1 hour)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	Isooctane	33%	13.0	6.5	µM product formed after 1 hour	0.1 M potassium phosphate buffer	7.4	37°C	100 µM Dextromorphan	Dextrophan	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 1 hour)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	Toluene	33%	13.0	0.0	µM product formed after 1 hour	0.1 M potassium phosphate buffer	7.4	37°C	100 µM Dextromorphan	Dextrophan	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 1 hour)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	Xylene	33%	13.0	0.0	µM product formed after 1 hour	0.1 M potassium phosphate buffer	7.4	37°C	100 µM Dextromorphan	Dextrophan	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 1 hour)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	Dichloromethane	25%	0.0	0.0	µM product formed after 2 hours	0.1 M potassium phosphate buffer	7.4	37°C	100 µM 7-benzyloxy-4-N,N-diethylamino-methyl-coumarin (BDAC)	7-benzyloxy-4-N-ethylaminomethyl-coumarin (BEAC)	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 2 hours)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	Cyclohexane	25%	0.0	0.09	µM product formed after 2 hours	0.1 M potassium phosphate buffer	7.4	37°C	100 µM 7-benzyloxy-4-N,N-diethylamino-methyl-coumarin (BDAC)	7-benzyloxy-4-N-ethylaminomethyl-coumarin (BEAC)	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 2 hours)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	n-Hexane	25%	0.0	0.1	µM product formed after 2 hours	0.1 M potassium phosphate buffer	7.4	37°C	100 µM 7-benzyloxy-4-N,N-diethylamino-methyl-coumarin (BDAC)	7-benzyloxy-4-N-ethylaminomethyl-coumarin (BEAC)	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 2 hours)
Activity + Stability - Product Formation	Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC	Isooctane	25%	0.0	0.13	µM product formed after 2 hours	0.1 M potassium phosphate buffer	7.4	37°C	100 µM 7-benzyloxy-4-N,N-diethylamino-methyl-coumarin (BDAC)	7-benzyloxy-4-N-ethylaminomethyl-coumarin (BEAC)	0.1 mM cumene hydroperoxide	200 rpm	Classical aqueous control (in microM product formed after 2 hours)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

Chloroform

33%

13.0

0.2

µM product formed after 1 hour

0.1 M potassium phosphate buffer

7.4

37°C

100 µM Dextromorphan

Dextrophan

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 1 hour)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

Dichloromethane

33%

13.0

0.0

µM product formed after 1 hour

0.1 M potassium phosphate buffer

7.4

37°C

100 µM Dextromorphan

Dextrophan

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 1 hour)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

1,1-Dichloroethane

33%

13.0

0.2

µM product formed after 1 hour

0.1 M potassium phosphate buffer

7.4

37°C

100 µM Dextromorphan

Dextrophan

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 1 hour)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

Butyl Ether

33%

13.0

2.3

µM product formed after 1 hour

0.1 M potassium phosphate buffer

7.4

37°C

100 µM Dextromorphan

Dextrophan

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 1 hour)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

Cyclohexane

33%

13.0

2.3

µM product formed after 1 hour

0.1 M potassium phosphate buffer

7.4

37°C

100 µM Dextromorphan

Dextrophan

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 1 hour)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

n-Pentane

33%

13.0

4.3

µM product formed after 1 hour

0.1 M potassium phosphate buffer

7.4

37°C

100 µM Dextromorphan

Dextrophan

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 1 hour)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

n-Hexane

33%

13.0

4.3

µM product formed after 1 hour

0.1 M potassium phosphate buffer

7.4

37°C

100 µM Dextromorphan

Dextrophan

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 1 hour)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

Isooctane

33%

13.0

6.5

µM product formed after 1 hour

0.1 M potassium phosphate buffer

7.4

37°C

100 µM Dextromorphan

Dextrophan

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 1 hour)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

Toluene

33%

13.0

0.0

µM product formed after 1 hour

0.1 M potassium phosphate buffer

7.4

37°C

100 µM Dextromorphan

Dextrophan

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 1 hour)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

Xylene

33%

13.0

0.0

µM product formed after 1 hour

0.1 M potassium phosphate buffer

7.4

37°C

100 µM Dextromorphan

Dextrophan

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 1 hour)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

Dichloromethane

25%

0.0

µM product formed after 2 hours

0.1 M potassium phosphate buffer

7.4

37°C

100 µM 7-benzyloxy-4-N,N-diethylamino-methyl-coumarin (BDAC)

7-benzyloxy-4-N-ethylaminomethyl-coumarin (BEAC)

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 2 hours)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

Cyclohexane

25%

0.0

0.09

µM product formed after 2 hours

0.1 M potassium phosphate buffer

7.4

37°C

100 µM 7-benzyloxy-4-N,N-diethylamino-methyl-coumarin (BDAC)

7-benzyloxy-4-N-ethylaminomethyl-coumarin (BEAC)

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 2 hours)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

n-Hexane

25%

0.0

0.1

µM product formed after 2 hours

0.1 M potassium phosphate buffer

7.4

37°C

100 µM 7-benzyloxy-4-N,N-diethylamino-methyl-coumarin (BDAC)

7-benzyloxy-4-N-ethylaminomethyl-coumarin (BEAC)

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 2 hours)

Activity + Stability - Product Formation

Measurement of the formed product after incubation (1 hour at 37°C) in the presence of organic solvent by HPLC

Isooctane

25%

0.0

0.13

µM product formed after 2 hours

0.1 M potassium phosphate buffer

7.4

37°C

100 µM 7-benzyloxy-4-N,N-diethylamino-methyl-coumarin (BDAC)

7-benzyloxy-4-N-ethylaminomethyl-coumarin (BEAC)

0.1 mM cumene hydroperoxide

200 rpm

Classical aqueous control (in µM product formed after 2 hours)

Visualization : Activity + Stability — Product Formation

Jin Zhao et al. (2007)

One bar per measurement. Colour = solvent, shade = solvent volume.

Jin Zhao et al. (2008) — The activity of human CYP2D6 in low water organic solvents

Biotechnology and Bioengineering · doi:10.1002/bit.22143 ↗ · Activity - Classical

16 measurements ▼

Database ID

UniProt: P10635 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host cells not mentioned

Experimental Data (16 measurements)

16 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	n-Hexane	Water-saturated organic solvent	100	11	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	0.2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	Cyclohexane	Water-saturated organic solvent	100	8	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	0.2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	Methylcyclohexane	Water-saturated organic solvent	100	14	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	0.2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	n-Heptane	Water-saturated organic solvent	100	9	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	0.2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	n-Octane	Water-saturated organic solvent	100	21	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	0.2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	Isooctane	Water-saturated organic solvent	100	55	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	0.2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	n-Decane	Water-saturated organic solvent	100	64	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	0.2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	n-Dodecane	Water-saturated organic solvent	100	61	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	0.2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	n-Hexane	Water-saturated organic solvent	100	4	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	Cyclohexane	Water-saturated organic solvent	100	5	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	Methylcyclohexane	Water-saturated organic solvent	100	5	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	n-Heptane	Water-saturated organic solvent	100	9	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	n-Octane	Water-saturated organic solvent	100	12	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	Isooctane	Water-saturated organic solvent	100	25	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	n-Decane	Water-saturated organic solvent	100	130	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme
Activity - Classical	Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)	n-Dodecane	Water-saturated organic solvent	100	101	%	0.1 M potassium phosphate buffer, 45 mM trehalose	7.4	37°C	0.05 mg Dextromethorphan	dextrorphan	2 mM cumene hydroperoxide	300 rpm	Classical aqueous control (in %) \| Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

n-Hexane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

0.2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

Cyclohexane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

0.2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

Methylcyclohexane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

0.2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

n-Heptane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

0.2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

n-Octane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

0.2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

Isooctane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

0.2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

n-Decane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

0.2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

n-Dodecane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

0.2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

n-Hexane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

Cyclohexane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

Methylcyclohexane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

n-Heptane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

n-Octane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

Isooctane

Water-saturated organic solvent

100

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

n-Decane

Water-saturated organic solvent

100

130

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Activity - Classical

Activity measured in the presence of organic solvent by HPLC (product formation after 2 hours at 37°C)

n-Dodecane

Water-saturated organic solvent

100

101

0.1 M potassium phosphate buffer, 45 mM trehalose

7.4

37°C

0.05 mg Dextromethorphan

dextrorphan

2 mM cumene hydroperoxide

300 rpm

Classical aqueous control (in %) | Lyophilized enzyme

Visualization : Activity — Classical

Jin Zhao et al. (2008)

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Cytochrome P450 2D6 from Homo sapiens

Enzyme Description

Sequence

Experimental Data (14 measurements)

Visualization : Activity + Stability — Product Formation

Experimental Data (16 measurements)

Visualization : Activity — Classical

Structure

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