Themolysin from Bacillus thermoproteolyticus

Enzyme Description

Species

Bacillus thermoproteolyticus ↗

Extremophile

Yes thermophilic organism

EC Number

3.4.24.27 ↗

Sequence

Length: 316 amino acids

ITGTSTVGVGRGVLGDQKNINTTYSTYYYLQDNTRGNGIFTYDAKYRTTLPGSLWADADNQFFASYDAPAVDAHYYAGVTYDYYKNVHNRLSYDGNNAAIRSSVHYSQGYNNAFWNGSQMVYGDGDGQTFIPLSGGIDVVAHELTHAVTDYTAGLIYQNESGAINEAISDIFGTLVEFYANKNPDWEIGEDVYTPGISGDSLRSMSDPAKYGDPDHYSKRYTGTQDNGGVHINSGIINKAAYLISQGGTHYGVSVVGIGRDKLGKIFYRALTQYLTPTSNFSQLRAAAVQSATDLYGSTSQEVASVKQAFDAVGVK

Hiroyasu Ogino et al. (2007) — Stabilities and Conformational Transitions of Various Proteases in the Presence of an Organic Solvent

Biotechnology Progress · doi:10.1021/bp060252p ↗ · Stability - Half-life

1 measurement ▼

Database ID

UniProt: P00800 ↗

Sequence Annotation

Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)

Protein Source

Commercially purchased

Experimental Data (1 measurement)

1 measurement

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Half-life	Half-life (in hours) at 30°C measured using absorbance spectrophotometry (free aromatic residues absorbance measurement, 280 nm)	Methanol	25%	416.0	98.1	hours	Incubation: 10 mM sodium phosphate buffer, Assay: 50 mM borax-HCl	Incubation: ?, Assay: 8.5	Incubation: 30°C, Assay: 30°C	0.6% (w/v) Casein	free amino acids , Peptides	—	—	Classical aqueous control (in hours)

Visualization : Stability — Half-life

Hiroyasu Ogino et al. (2007)

One bar per measurement. Colour = solvent, shade = solvent volume.

Akli Ouelhadj et al. (2020) — Identification and homology modeling of a new biotechnologically compatible serine alkaline protease from moderately halotolerant Gracilibacillus boraciitolerans strain LO15

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.07.266 ↗ · Activity - Classical

10 measurements ▼

Database ID

UniProt: P00800 ↗

Sequence Annotation

Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)

Protein Source

Commercially purchased

Experimental Data (10 measurements)

10 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Decane	25%	100	90	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Octane	25%	100	132	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Hexane	25%	100	101	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	25%	100	92	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	25%	100	66	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	25%	100	15	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Isopropanol	25%	100	91	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Acetonitrile	25%	100	0	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Methanol	25%	100	75	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100	127	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Decane	25%	100	90	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Octane	25%	100	132	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Hexane	25%	100	101	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	25%	100	92	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	25%	100	66	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	25%	100	15	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Isopropanol	25%	100	91	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Acetonitrile	25%	100	0	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Methanol	25%	100	75	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100	127	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

Akli Ouelhadj et al. (2020)