Benzaldehyde lyase from Pseudomonas fluorescens
Enzyme Description
Sequence
MAMITGGELVVRTLIKAGVEHLFGLHGAHIDTIFQACLDHDVPIIDTRHEAAAGHAAEGYARAGAKLGVAGHGGRGIYQCGHAHCQRLAGSQGRCIPHPGSGALRDDETNTLQAGIDQVAMAAPITKWAHRVMATEHIPRLVMQAIRAALSAPRGPVLLDLPWDILMNQIDEDSVIIPDLVLSAHGARPDPADLDQALALLRKAERPVIVLGSEASRTARKTALSAFVAATGVPVFADYEGLSMLSGLPDAMRGGLVQNLYSFAKADAAPDLVLMLGARFGLNTGHGSGQLIPHSAQVIQVDPDACELGRLQGIALGIVADVGGTIEALAQATAQDAAWPDRGDWCAKVTDLAQERYASIAAKSSSEHALHPFHASQVIAKHVDAGVTVVADGALTYLWLSEVMSRVKPGGFLCHGYLGSMGVGFGTALGAQVADLEAGRRTILVTGDGSVGYSIGEFDTLVRKQLPLIVIIMNNQSWGATLHFQQLAVGPNRVTGTRLENGSYHGVAAAFGADGYHVDSVESFSAALAQALAHNRPACINVAVALDPIPPEELILIGMDPFA
Elena Janzen et al. (2006)
β Characterization of benzaldehyde lyase from Pseudomonas fluorescens: A versatile enzyme for asymmetric CβC bond formation
Bioorganic Chemistry
Β· doi:10.1016/j.bioorg.2006.09.002 β
Β· Activity + Stability - Product Formation
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Database ID
UniProt: P51853 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli SG13009
Experimental Data (2 measurements)
2 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Product Formation | Product benzoin formation after 1 hour incubation in the presence of organic solvent at 30Β°C, measured by HPLC | Dimethyl Sulfoxide (DMSO) | 20% | 0.45 | 0.42 | mM | 50 mM potassium phosphate buffer, 5 mM Mg2+ | 7 | 30Β°C | 50 mM Benzaldehyde | Benzoin | 0.1 mM ThDP | β | Classical aqueous control (in mM) |
| Activity + Stability - Product Formation | Product benzoin formation after 1 hour incubation in the presence of organic solvent at 30Β°C, measured by HPLC | PEG-400 | 15% | 0.45 | 0.54 | mM | 50 mM potassium phosphate buffer, 5 mM Mg2+ | 7 | 30Β°C | 50 mM Benzaldehyde | Benzoin | 0.1 mM ThDP | β | Classical aqueous control (in mM) |
Visualization : Activity + Stability β Product Formation
One bar per measurement. Colour = solvent, shade = solvent volume.