Vanadium-dependent haloperoxidase from Corallina officinalis

Enzyme Description

Species
Corallina officinalis β†—
Extremophile
No
EC Number
1.11.1.10 β†—

Sequence

Length: 598 amino acids
MGIPADNLQSRAKASFDTRVSAAELALARGVVPSLANGEELLYRNPDPENGDPSFIVSFTKGLPHDDNGAIIDPDDFLAFVRAINSGDEKEIADLTLGPARDPDTGLPIWRSDLANSLELEVRGWENSSAGLTFDLEGPDAQSIAMPPAPVLTSPELIAEIAELYLMALGREIEFSEFDSPKNAEYIQFAIDQLNGLEWFNTPAMLGDPPAEIRRRRGEVTVGNLFRGILPGSEVGPYLSQYIIVGSKQIGSATGGNKTLVSPNAADEFDGEIAYGSITISQRVRIATPGRDFMTDLKVFLDVQDAADFRGFESYEPGARLIRTIRDLATWVHFDALYEAYLNACLILLANRVPFDPNIPFQQEDKLDNQDVFVNFGDAHVLSLVTEVATRALKAVRYQKFNIHRRLRPEATGGLISVNKIAAEKGESVFPEVDLAVEELEDILEKAEISNRKQNIADGDPDPDPSFLLPQAFAEGSPFHPSYGSGHAVVAGACVTILKAFFDSNFQIDQVFEVDKDEDKLVKSSFKGTLTVAGELNKLADNIAIGRNMAGVHYFSDQFESILLGEQVAIGILEEQSLTYGENFFFNLPKFDGTTIQI
E.E. Coupe et al. (2007) β€” The dodecameric vanadium-dependent haloperoxidase from the marine algae Corallina officinalis: Cloning, expression, and refolding of the recombinant enzyme
Protein Expression and Purification  Β· doi:10.1016/j.pep.2006.08.010 β†—  Β· Activity - Classical
16 measurements ▼
Database ID
UniProt: Q8LLW7 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (HOBr and Chlorodimedone reaction product absorbance measurement, 290 nm) in the presence of organic solvent Ethanol 20% 100 92 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Ethanol 40% 100 89 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Ethanol 60% 100 82 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Ethanol 80% 100 43 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Isopropanol 20% 100 57 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Isopropanol 40% 100 65 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Isopropanol 60% 100 87 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Isopropanol 80% 100 104 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Methanol 20% 100 97 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Methanol 40% 100 89 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Methanol 60% 100 86 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Methanol 80% 100 13 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Acetonitrile 20% 100 123 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Acetonitrile 40% 100 122 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Acetonitrile 60% 100 120 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry Acetonitrile 80% 100 103 % 50 mM MES buffer, 0.1 MM Chlorodimedone 6.5 25Β°C Bromide ion, 1 mM Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) H2O , HOBr β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*